作　　者： ; ; ;

机构地区： 遵义师范学院生物系

出　　处： 《西南师范大学学报（自然科学版）》 2012年第6期83-86,共4页

摘　　要： 利用常用细菌分离纯化方法从患病草鱼中分离出7株菌株,分别编号为Ⅰ～Ⅶ;对7株菌株的16SrRNA基因序列进行PCR扩增,均得到长约600bp的片段;将扩增结果进行测序,测序结果输入到美国国立生物技术信息中心(NCBI,http://www.ncbi.nlm.nih.gov/),用BLAST工具对序列进行同源性比对分析.结果表明:Ⅰ为沙雷氏菌,同源性达95%;II为腐生葡萄球菌,同源性达99%;III为荧光假单孢菌,同源性达97%;IV为产碱普罗威登斯菌,同源性达99%;V为嗜水气单孢菌,同源性达99%;VI为杀鲑气单孢菌,同源性达97%;VII为不动菌属,同源性达97%.将Ⅰ～Ⅶ号菌株回复感染健康草鱼,结果沙雷氏菌、腐生葡萄球菌、荧光假单孢菌、产碱普罗威登斯菌和嗜水气单孢菌对草鱼的危害较大,而杀鲑气单孢菌和不动菌属的感染症状不明显. In the present experiment,the traditional microbiology method was used to isolate the endophytic bacteria from Lateolabrax japonicus of net cage culture in the Wujiang River.A total of 7 strains were isolated.Their 16S rRNA gene sequences were amplificated by PCR,and 600 bp fragments were obtained.These 600 bp fragments were successfully sequenced and entered into the NCBI（http： //www.ncbi.nlm.nih.gov /）.Alignment analysis was made by BLAST tool to analyse the homology.The results showed that the 7 stains were Serratia proteamaculans（homology of 95%）,Staphylococcus saprophyticus（homology of 99%）,Pseudomonas fluorescens（homology of 97%）,Providencia alcalifaciens（homology of 99%）,Aeromonas hydrophila（homology of 99%）,Aeromonas salmonicida（homology of 97%）,and Acinetobacter（homology of 97%）.The bacteria were used for retrieved infection of the healthy C.idellus.S.proteamaculans,S saprophyticus,P.fluorescens,P.alcalifaciens and A.hydrophila were shown to have comparatively large hazard to grass carp,while A.salmonicida,and Acinetobacter induced no obvious infection.

关 键 词： 草鱼 细菌 分离 分子鉴定

领　　域： [生物学]