作　　者： ; ; ; (吴咚咚）; (陈颖）;

机构地区： 福州市农业科学研究所福建福州350018

出　　处： 《生物安全学报》 2012年第2期109-113,共5页

摘　　要： 【背景】烟粉虱作为世界性的多食性害虫,对我国农业生产造成了严重危害。已有研究表明,许多植物的次级代谢产物能够对烟粉虱解毒酶和靶标酶活性产生影响。【方法】采用常规生化方法研究了辣椒碱对烟粉虱体内羧酸酯酶(CarE)、谷胱甘肽S-转移酶(GSTs)和乙酰胆碱酯酶(AChE)活性的影响。【结果】与对照相比,500mg·L-1辣椒碱处理烟粉虱成虫后6h,CarE活性被激活,其他时段被抑制;1000mg·L-1辣椒碱处理后6、12、48h,CarE活性受到抑制,其他时段被激活;2000mg·L-1辣椒碱处理后6h,CarE活性受到抑制,其他时段被激活;4000mg·L-1辣椒碱处理后3、12h,CarE活性受到抑制,其他时段被激活;8000mg·L-1辣椒碱处理后1h,CarE活性被激活,其他时段受到抑制。辣椒碱对烟粉虱体内GSTs活性的诱导存在剂量和时间效应,8000mg·L-1辣椒碱处理烟粉虱成虫后24和48h,GSTs活性受到明显抑制,其余各处理在任何时段GSTs活性均被激活而高于对照。辣椒碱各浓度处理对烟粉虱体内AChE活性的诱导存在时间效应,各处理均在处理后48h对AChE活性产生了抑制作用。【结论与意义】辣椒碱对烟粉虱体内的解毒酶CarE和GSTs、靶标酶AChE活性具有明显影响,且存在剂量或时间效应,这可为辣椒碱在生物农药领域的应用提供理论依据。 [ Background] The sweet potato whitefly, Bemisia tabaci Gennadius, is one of the most important pests worldwide, and has caused serious damage to agriculture production in China. Plant secondary metabolites may significantly affect the activity of sev- eral enzymes involved in detoxification, including carboxylesterase （ CarE）, glutathione S-transferase （GSTs） and acetylcholinester- ase （ACHE） in B. tabaci. [ Method]The effects of capsaicin on activities of CarE, GSTs and AChE in B. tabaci were tested by con- ventional biochemical methods. [ Result ] After exposed to 500 mg·L^-1 capsaicin; CarE was activated at 6 h, but was inhibited at other times when the capsaicin concentration was increaed to 1000 mg·L^-1, the activity was inhibited at 6 h, 12 h and 48 h, but was activated at other times; at 2000 mg·L^-1, the activity was inhibited at 6 h, but was activated at other times. Treatment with 4000 mg·L^-1 capsaicin caused inhibition at 3 h and 12 h, but activation at other times; at 8000 mg·L^-1, the enzyme was activa- ted at 1 h, but inhibited at other times. At a concentration of 8000 mg·L^-1, the activity of GSTs was inhibited significantly at 24 h and 48 h, but was activated at other times and other concentrations. In the case of AchE, the activity inhibition happened at 48 h after every concentration of capsaicin application. [ Conclusion and significance ] The capsaicin may significantly affect the enzyme activities of CarE, GSTs and AChE in B. tabaci with the dose- or time-dependent effects. This study could provide a basis for capsai- cin application against B. tabaci.

关 键 词： 辣椒碱 烟粉虱 入侵昆虫 羧酸酯酶 谷胱甘肽 转移酶 乙酰胆碱酯酶 生物防治

领　　域： [生物学]