作　　者： ; ; ; ; ; ; ;

机构地区： 暨南大学医学院

出　　处： 《中国病理生理杂志》 2012年第6期974-979,共6页

摘　　要： 目的:利用原子力显微镜在纳米级分辨率下观察药物对肿瘤细胞膜表面形貌结构的影响。方法:采用不同浓度的抗肿瘤药物青蒿琥酯作用于人胃癌细胞株SGC-7901,24 h后运用原子力显微镜观察细胞膜表面形貌、三维结构及超微结构的变化。流式细胞术检测细胞凋亡情况。结果:对照组细胞核区饱满,膜表面平坦光滑;加药组细胞核区塌陷,萎缩,膜表面形成了孔洞和凹陷;且随青蒿琥酯浓度的增加,孔洞直径加大,数量增多。超微结构观察到对照组细胞膜表面颗粒较密集,有的呈条索状,有的聚集成团,堆积较为紧凑;而加药组细胞膜表面有陷窝,颗粒稀少,分布较疏松。膜超微结构参数分析显示,加药组细胞核区高度降低,膜微区的平均粗糙度(Ra)和均方粗糙度(Rq)均降低。经流式细胞术分析,加药组细胞凋亡率随青蒿琥酯浓度的增加而增大,与对照组相比均有显著差异。结论:本实验结果不仅有助于寻找细胞特异性的形貌学改变,也为揭示抗癌药物的作用机制提供微观形态学参考。 AIM： To detect the membrane surface morphology of cancer cells treated with artesunate （ART） under atomic force microscope （AFM）. METHODS： Human gastric cancer SGC-7901 cells were cultured and treated with different concentrations of ART for 24 h. The membrane surface morphology, three-dimensional structures and ultrastructural changes of SGC-7901 cells were observed under AFM. The apoptosis of SGC-7901 cells was detected by flow cytometry. RESULTS： The AFM images revealed that the cell nuclear area was full and the surface of cell membrane was flat and smooth in control group. Compared with control group, the cell nuclear area collapsed and suffered atrophy, and the membrane surface had more pores in ART treatment groups. More pores were observed and the diameter of the pores was increased with the increase in ART concentration. The cell membrane ultrastructure showed that the particles in control group had an intensive distribution, some were cord-shaped and some gathered into a mass. The particles in ART treatment groups were fewer and distributed sparsely. Moreover, there were obvious lacunae in the surface of cell membrane. Quantitive measurement found that the height of cell nucleus area was decreased, and the surface root mean square roughness （Rq） and the surface average roughness （Ra） became smaller in ART treatment groups compared with control group. The apoptotic rates of the cells in ART treatment groups were increased with the increase in ART concentration, and were all significantly higher than that in control group. CONCLUSION： Our findings emphasize the significance of AFM in exploring the changes of the membrane surface morphology at nano-scale resolution following the treatment with anticancer drugs, which could not only identify the specific characteristics of morphological changes of the tumor cells, but also provide micromorphological references to reveal the mechanisms of anticancer drugs.

关 键 词： 原子力显微镜 形态结构 青蒿琥酯

领　　域： [生物学]