作　　者： ; ; ; ;

机构地区： 广东省农业科学院

出　　处： 《中国农业科学》 2012年第10期2093-2098,共6页

摘　　要： 【目的】明确广东省果园或菜田田埂牛筋草(Eleusine indica)对草甘膦(glyphosate)的抗药性水平。【方法】利用整株测定法测定广东省广州市、惠州市、梅州市等地共7个点牛筋草对草甘膦的抗药性水平;采用紫外分光光度计测定抗性水平差异最大的2种牛筋草体内莽草酸含量的差异;采用光纤型双通道PAM-100测定抗性水平差异最大的2种牛筋草叶片各自叶绿素荧光参数的差异。【结果】惠州市杨村番石榴园(以下简称杨村)牛筋草对草甘膦的相对抗性指数达11.0,试验结果与田间实际反映情况相吻合;莽草酸含量测定结果表明,1 845g a.i./hm2草甘膦处理后0—7 d,杨村牛筋草植株内部莽草酸含量较低,与对照相当,而广州番禺牛筋草植株内部积累了较多莽草酸,草甘膦处理后7 d,其莽草酸含量(623.1g·g FW-1)为杨村牛筋草(68.1g·g FW-1)的9.1倍;叶片荧光参数结果表明,杨村牛筋草Fv/Fm(PSⅡ最大原初光能转化效率)、α(PSII光响应曲线的初始斜率,代表了植物对光能的利用效率)值均高于广州番禺牛筋草,且值较稳定,而广州番禺牛筋草Fv/Fm和α在草甘膦处理5 d后均降为0。【结论】广东省部分果园或菜田田埂牛筋草对草甘膦已产生不同程度的抗药性,其中杨村牛筋草抗性最高;杨村牛筋草体内积累的莽草酸很低,植株光合系统基本未受到影响,光合作用正常进行。 【Objective】The objective of this study is to investigate the resistance level of Eleusine indica to glyphosate,a 5-enolpyruvylshikimate-3-phosphate（EPSP） synthase inhibiting herbicide.【Method】Seven E.indica populations were collected from different orchards or ridges of different areas in Guangzhou,Huizhou,Meizhou in Guangdong Province where glyphosate had been continuously applied for different years.The resistance level of E.indica to glyphosate was assessed by whole plant assay.The differences of shikimic acid content and chlorophyll fluorescence parameters（Fv/Fm and α） in the most resistant and susceptible populations were measured by ultraviolet spectrophotometer and Dual PAM-100,respectively.【Result】The relative resistance index of E.indica population which collected from Yangcun in Huizhou was 11.0.The result obtained in laboratory was agreed with the real fact in fields.Furthermore,E.indica collected from Yangcun accumulated lower level shikimic acid in 7 days after glyphosate treatment,while that one collected from Panyu in Guangzhou was the most susceptible population accumulated 9.1 times shikimic acid of Yangcun population on 7 th after 1 845 g a.i./hm2 of glyphosate application.The results from the analysis of chlorophyll fluorescence parameters showed that the values of Fv/Fm and α in leaves of E.indica from Yangcun were generally higher than that one from Panyu in Guangzhou,and the values of both parameters in leaves of E.indica from Panyu were declined to zero on 5 th after glyphosate treatment.【Conclusion】 E.indica which collected from orchard or ridge has apparently evolved resistance to glyphosate,and Yangcun population is the most resistant one,which accumulated lower level of shikimate acid and photosynthesis system worked well basically after glyphosate treatment.

关 键 词： 牛筋草 草甘膦 抗性水平 叶绿素荧光

领　　域： [农业科学] [农业科学]