作　　者： ; ; ; ; ; ; ; ; ; ; ;

机构地区： 南方医科大学基础医学院

出　　处： 《中国病理生理杂志》 2012年第5期878-883,共6页

摘　　要： 目的:观察SETD4(SET domain containing 4)蛋白在不同细胞中的表达和定位情况,以及亚砷酸钠(NaAsO2)刺激对细胞SETD4表达和定位的影响,探讨NaAsO2诱导SETD4的改变是否依赖于p38信号通路。方法:用Western blotting检测SETD4在不同细胞中蛋白的表达情况,用细胞免疫荧光技术检测SETD4在细胞中的定位;用NaAsO2刺激p38+/+和p38-/-细胞,分别收集细胞总蛋白检测SETD4蛋白的表达变化,同时观察SETD4的定位和移位改变。结果:SETD4蛋白无论在鼠源还是人源的细胞中均有表达;细胞免疫荧光结果显示SETD4在整个细胞中分布,以胞质较多;p38+/+和p38-/-细胞受NaAsO2刺激后其SETD4蛋白的表达趋势一致,即均在6 h时有明显减少;p38+/+细胞受NaAsO2刺激后SETD4蛋白从胞质向胞核移位,而p38-/-细胞SETD4的移位现象不明显。结论:SETD4蛋白在不同种属及组织来源的细胞中均有表达,表现为全细胞分布,以胞质为主;NaAsO2刺激可影响细胞SETD4蛋白的表达水平及定位,NaAsO2诱导的SETD4移位改变有赖于p38 MAPK介导的信号通路。 AIM ： To investigate the expression and location of SET domain - containing 4 （SETD4） protein in p38＋/＋ and p38-/- cells treated with sodium arsenite（ NaAsO2 ）. METHODS： The expression and location of SETD4 were detected in different cells with or without NaAsO2 treatment by Western blotting and technique. RESULTS： The expression of SETD4 was detectable in both murine - and human -derived ceils. Its distribution was found to be located in the whole cell, mainly in the cytoplasm. Further investigation also suggested that the protein expres- sion of SETD4 was reduced in both p38~/~ and p38-/- cells 6 h after NaAs02 treatment. Moreover, SETD4 protein was translocated from cytoplasm to nucleus in p38 ~/＋ ceils treated with NaAs02, which was unobvious in p38-/- cells. CON- CLUSION： SETD4 protein is expressed in various cells derived from different species and tissues, and it is mainly located in cytoplasm. NaAsOz treatment influences the expression of SETD4, and induces the translocation of SETD4 protein to nu- cleus, which might be involved in the p38 MAPK signal pathway.

关 键 词： 家族蛋白 蛋白 亚砷酸钠 通路

领　　域： [生物学]