作　　者： ; ; ; ; ;

机构地区： 长春工业大学化学与生命科学学院

出　　处： 《吉林大学学报（理学版）》 2012年第3期581-586,共6页

摘　　要： 利用噬菌体肽库筛选与计算机模拟分子对接技术,优化异柠檬酸裂解酶肽类抑制剂的筛选.先通过噬菌体肽库筛选出与异柠檬酸裂解酶(ICL)具有高亲和力的结合肽,再利用Discovery Studio 2.1模拟多肽与ICL蛋白晶体(1F8I)的分子对接,最后用Fmoc固相合成法合成多肽,并对其生物活性进行检测.实验结果表明,通过噬菌体肽库筛选得到了29条七肽序列,其中12条可与ICL蛋白晶体成功对接.体外生物活性检测结果显示,得到的12条七肽均对ICL的活性有明显抑制作用(抑制率均超过50%). Using the computer simulation technology of docking and the phage peptide library for optimum screening of the isocitrate lyase peptide inhibitor.First the binding peptides were screened by phage peptide library,which has a high affinity for isocitrate lyase（ICL）.And then via the Discovery Studio 2.1,the docking of peptides to ICL protein crystal（1F8I） was simulated.Finally,the solid-phase synthesis of Fmoc was used to synthesize peptide,and their biological activities were detected.We got 29 heptadpeptide sequences by phage peptide library screening,12 of which can successfully dock to ICL protein crystal.The biological activity detecting shows that they all have conspicuous inhibition on the ICL activity（rate of inhibition 50%）.

关 键 词： 异柠檬酸裂解酶 肽类抑制剂 噬菌体肽库 虚拟筛选 分子对接

领　　域： [生物学]