作　　者： ; ; ; ; ; ; ;

机构地区： 云南农业大学动物科学技术学院云南省动物营养与饲料重点实验室

出　　处： 《动物营养学报》 2011年第11期1946-1952,共7页

摘　　要： 本试验旨在研究饲粮不同能量水平对乌金猪脂肪组织脂类分解代谢相关基因表达的影响。选取体重约15kg的乌金猪54头,随机分为3组,每组3个重复,每个重复6头猪,分别饲喂消化能为11.74(低能组)、12.89(中能组)和14.22M J/kg(高能组)的饲粮,在体重30、60和100kg时屠宰取皮下脂肪组织,提取总RNA,荧光定量PCR法检测脂肪组织脂肪分解代谢相关酶和因子[激素敏感脂肪酶(HSL)、肉碱脂酰转移酶Ⅰ(CPT-Ⅰ)、脂蛋白脂酶(LPL)和过氧化物酶体增殖物激活受体γ(PPARγ)]基因的表达水平。结果表明:饲喂高能量饲粮乌金猪在30和100kg体重时,脂肪组织HSL、CPT-Ⅰ和PPARγ基因的表达水平显著下降(P<0.05),LPL基因的表达水平显著升高(P<0.05);在60kg体重时,4种基因的表达水平均显著升高(P<0.05)。结果提示,高能量饲粮可降低乌金猪在30和100kg体重时脂肪的分解和脂肪酸的β-氧化,增强60kg体重时脂肪的分解和脂肪酸的β-氧化。 The aim of this study was to investigate the effects of dietary energy level on the expression of lipid catabolism-related genes in adipose tissue of Wujin pigs.Fifty-four Wujin pigs with body weight of 15 kg were randomly assigned into 3 groups with 3 replicates per group and 6 heads in each replicate.Pigs in low digestive energy group（11.74 MJ/kg,LDE group）,middle digestive energy group（12.89 MJ/kg,MDE group） and high digestive energy group（14.22 MJ/kg,HDE group） were fed diets with three different digestive energy levels.At the body weight of 30,60 and 100 kg,pigs were slaughtered and subcutaneous adipose tissue was collected for analysis of gene expression levels of lipid catabolism-related enzymes and factors [hormone-sensitive lipase（HSL）,carnitine acyl transferase Ⅰ（CPT-Ⅰ）,lipoprotein lipase（LPL） and preoxisome proliferator-activated receptor γ（PPARγ）] by real-time PCR.The results showed as follows： at the body weight of 30 and 100 kg,the gene expression levels of HSL,CPT-Ⅰ and PPARγ in adipose tissue of Wujin pigs fed high energy level diet significantly decreased（P0.05）,while the gene expression level of LPL significantly increased（P0.05）;however,at the body weight of 60 kg,the expression levels of the four genes were significantly increased（P0.05）.The results indicate that high dietary energy level can decrease lipid catabolism and fatty acid β-oxadation of Wujin pigs at the body weight of 30 and 100 kg,but enhance lipid catabolism and fatty acid β-oxadation of Wujin pigs at the body weight of 60 kg.

关 键 词： 乌金猪 饲粮能量水平 脂肪组织 脂肪分解代谢 基因表达

领　　域： [农业科学] [农业科学]