机构地区: 厦门大学生命科学学院
出 处: 《中国细胞生物学学报》 2012年第4期361-365,共5页
摘 要: 该文用酶解一振荡法分离蝴蝶兰受精后的胚囊,然后显微解剖出合子、早期原胚及接近成熟的球形原胚。酶解液由O.7%-1.3%的纤维素酶、0.6%-1.0%果胶酶和10%甘露醇组成,pH5.8,酶解时间20-30min。分离的蝴蝶兰早期原胚和接近成熟的球形原胚均发现有发达的胚柄吸器。 Zygotes, proembryos and maturing spherical proembryos were isolated from Phalaenopsis hybrid embryo sacs by the method of manual microdissection after a pretreatment of combinnating enzymatic mac- eration with shaking the ovules. The enzymatic maceration was under 0.7%-1.3% cellulase, 0.6%-1.0% pectinase, 10% mannitol, pH5.8 for about 20-30 min. Developed suspensor haustorium was discovered on the micro-dissect- ed embryos from three-cell stage to maturing spherical.