作　　者： ; ; ; ; ; ; ; ; ;

机构地区： 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室

出　　处： 《中国预防兽医学报》 2012年第3期223-226,共4页

摘　　要： 牛分枝杆菌(M.bovis)是引起牛结核病的常见病原,而鸟分枝杆菌(M.avium)2型则通常交叉感染牛,但不会导致严重的病变。为鉴定M.bovis和M.avium的免疫交叉反应情况,本研究分别以M.bovis和M.avium2型的基因组为模板,扩增ag85b基因,分别构建了M.bovis和M.avium的原核和真核表达重组质粒进行表达。以原核表达纯化的两种重组蛋白Ag85b(rAg85b)分别作为包被抗原,交叉检测两种真核重组质粒免疫豚鼠制备的抗血清。结果表明,原核表达的M.bovis和M.avium rAg85b与真核重组质粒免疫豚鼠制备的两种抗血清之间存在较强的免疫交叉反应。研究结果揭示M.bovis和M.avium的Ag85b蛋白存在很强的血清学交叉反应,这将严重干扰M.bovis Ag85b作为候选疫苗的免疫监测。 Mycobacterium bovis is a common pathogen to cause bovine tuberculosis in cattle, but crossing infection of Mycobacterium avium is the normal cases in cattle. To identify serum crossing reactions of the 2 bacteria, the ag85b genes were amplified by PCR from genomes of M. boris and M. avium serotype 2, respectively, and subcloned into pET28a and pcDNA3.1 （pcDNA-Ag85b）. Guinea pigs were immunized with pcDNA-Ag85b of M. bovis and M. avium, respectively, and the prepared anti-Ag85bs were used to analyze the cross-reactions of the two different Mycobacteda by ELISA with recombinant Ag85b proteins as coating antigens expressed in E. coli. The results shown that M. boris and M. avium had strong cross-reactions between the two kinds of Ag85b proteins, which provided evidence that M. avium infection would interfere the immunologic surveillance to the use of vaccines containing Ag85b in cattle herd.

关 键 词： 牛结核分枝杆菌 鸟型分枝杆菌 基因 交叉反应

领　　域： [农业科学] [农业科学] [农业科学]