作　　者： ; ; ; ; ; ; ; ; ;

机构地区： 军事医学科学院放射与辐射医学研究所

出　　处： 《解放军医学杂志》 2012年第4期379-382,共4页

摘　　要： 目的探讨微波辐射对睾丸组织磷酸化环磷酸腺苷(cAMP)-反应元件结合蛋白(pCREB)、cAMP反应元件调节因子(CREM)及CREB结合蛋白(CBP)表达的影响及其意义。方法将30只雄性Wistar大鼠随机分为对照组(n=5)和辐射组(n=25)。辐射组接受30mW/cm2微波辐射5min,分别于辐射后6h,1、3、7、14d处死5只大鼠。对照组不接受辐射,于1d时活杀。采用免疫组织化学及Western blotting检测睾丸组织pCREB、CREM及CBP蛋白表达的变化。结果 pCREB、CREM和CBP均主要表达于睾丸生精小管精子细胞核。微波辐射后6h^14d(pCREB在1d时除外),pCREB和CBP蛋白表达明显下调(P<0.05或P<0.01),辐射后6h^7d CREM表达亦明显下调(P<0.05或P<0.01)。结论 pCREB、CREM及CBP表达下调在微波辐射致生精细胞损伤中可能发挥重要作用。 Objective To investigate the effects and ascertain the significance of microwave radiation on the expression of phosphorylated cyclic adenosine monophosphate（cAMP）-response element-binding protein（pCREB）,cAMP-responsive element modulator（CREM）,and CREB-binding protein（CBP） in the testicular tissue of rats.Methods Thirty male Wistar rats were randomly divided into control group（n=5） and radiation group（n=25）.Five rats in the radiation group were sacrificed at 6h,1,3,7,and 14d,respectively,after exposure to microwave radiation for 5min,with an average power density of 30mW/cm2.Rats in the control group were sacrificed within 1d without receiving microwave radiation.Expressions and dynamic changes in pCREB,CREM,and CBP in the testicular tissues were examined by immunohistochemistry and Western blotting.Results pCREB,CREM,and CBP were mainly expressed in the sperm nuclei of the seminiferous tubule in the rat testis.pCREB and CBP protein expressions were downregulated from 6h to14d（except for pCREB at 1d） after exposure to microwave radiation（P0.05 or P0.01）.The expression of CREM was also weakened significantly from 6h to 7d after radiation（P0.05 or P0.01）.Conclusion The downregulation of pCREB,CREM,and CBP expression may play an important role in the injury of spermatogenic cells caused by microwave radiation.

关 键 词： 微波 辐射损伤 实验性 反应元件结合蛋白质 应答元件调节器 结合蛋白质 睾丸 大鼠

领　　域： [生物学] [生物学]