机构地区: 贵州大学生命科学学院
出 处: 《中国酿造》 2012年第3期35-38,共4页
摘 要: 从酱香型酒大曲中分离得到一株白地霉MTBD,对其进行发酵实验,检测蛋白酶活力,测定其在不同原料比、不同培养时间、不同pH值、不同接种量、不同培养温度、不同料水比条件下产蛋白酶的情况,同时对其产酶条件进行优化,获得最佳产酶条件为:培养温度为45℃、pH值为3.5、料水比为1∶1、培养时间为6d。 A Geotrichum candidum Lnak MTBD was isolated from Daqu of Moutai-flavor liquor.The fermentation experiments were carried out for detection of protease activity under in the conditions of different raw materials,different fermentation time,different pH,different inoculum,different culture temperature,different ratios of material to water.And the enzyme production conditions were optimized,as well,which was as follows: culture temperature 45℃,pH 3.5,ratio of material to water 1∶1,fermentation time 6d.