作　　者： ; ; ; ; ; ; ; (张兴波）;

机构地区： 国家林业局

出　　处： 《西部林业科学》 2012年第1期1-6,共6页

摘　　要： 通过对浦竹仔红秆变异个体基因组DNA的提取、rDNA ITS片段的扩增、回收及测序,分析了其rDNA ITS序列,构建了系统发育树。结果表明,供试竹种与Genbank中记录的浦竹仔ITS全长序列相似度为99%,与同属的中华大节竹、酸竹属的酸竹、少穗竹属的少穗竹亲缘关系较近,其遗传距离为0.007和0.010。RNA二级结构模拟分析显示,各参试竹种之间5.8s RNA变异极微小,主要的遗传变异均集中在ITS1-ITS2非编码序列中。由此可以推论该竹种产花色苷性状并非种属差异导致,为样本收集地环境因子诱发基因突变所致。 Through DNA extraction, PCR amplification and DNA sequencing, the internal transcribed spacers （ITS） sequence of anthocyanin produced mutant of Indosasa hispida were analyzed and the phylogenetic tree was established. The results indicated that the ITS sequence similarity between the tested materials and the record of Indosasa hispida was 99 % , anthocyanin produced mutant had close genetic relationship with Indosasa siniea, Acido- sasa purpurea and Oligostachyum sulcatum, with the genetic distance of 0. 007 and 0. 010 respectively. RNA secondary structure simulation analysis showed the genetic variation in 5.8 sRNA was extremely small, the main genetic variation were in ITS1 -ITS2 noncoding sequence. The results therefore implied that the anthocyanin produced character was not the genus or species difference, but caused by environmental mutagenesis.

关 键 词： 浦竹仔 变异个体 序列 系统发育

领　　域： [农业科学] [农业科学]