作　　者： ; ; ; ;

机构地区： 佛山科学技术学院理学院

出　　处： 《食品科学》 2012年第3期48-53,共6页

摘　　要： 以波纹巴非蛤为原料,酶解1～6h制备酶解液。在SDS-PAGE电泳法测定肽谱的基础上,再利用液相色谱-质谱联用测定波纹巴非蛤酶解的肽谱变化。结果表明:酶解前3h时酶解液的肽分子的分子质量大概主要集中在10 0、5 0、2 kD,酶解液大都是大分子肽或者中分子肽;酶解4、5、6 h时酶解液的肽分子的分子质量基本没变化,其分子质量主要集中在400～600D,即为小分子肽,酶解4h时为酶解终点。 Paphia undulate muscles were hydrolyzed with papaln and the hydrolysates after hydrolysis for 1, 2, 3, 4, 5 h and 6 h were analyzed by SDS-PAEG and LC-MS/MS. The results revealed that the molecular mass distribution of peptides in Paphia undulate muscle hydrolysates was mostly concentrated on 100, 50 kD and 2 kD during the firSt 3 hours of the hydrolysis, most of them large or small peptides. No substantial difference in the molecular mass distribution of peptides, which was mostly between 400 D and 600 D, was observed among the 4 h, 5 h and 6 h hydrolysates, suggesting that 4 h was the end point of the hydrolysis.

关 键 词： 波纹巴非蛤 活性肽 电泳法 液相色谱 质谱联用

领　　域： [生物学]