作　　者： ; ;

机构地区： 华南农业大学动物科学学院动物营养与饲料科学系

出　　处： 《华南农业大学学报》 2000年第1期75-77,共3页

摘　　要： 用ＩＢＤＶ抗体与某ＩＢＤ商品疫苗制成ＩＢＤＶ－Ａｂ复合苗．用ＩＢＤＶ－Ａｂ复合苗与ＩＢＤ商品疫苗在有母源抗体的雏鸡中进行免疫对比试验．试验鸡随机分为４组，饲养在隔离器中　第１组为空白对照组，第２组为攻毒对照组，第３组在 １０ ｄ龄用复合苗进行免疫，第 ４组在 １０ ｄ龄用商品苗进行免疫．各组从 １１ｄ龄起每隔 １０ ｄ采血，用酶联免疫吸附试验检测血清中 ＩＢＤ抗体水平，３５ ｄ龄时进行攻毒试验．试验结果显示，２１ｄ龄时，第３、４组的抗体水平分别为７３８、９４６；３１ｄ龄时，分别为 ２ ４０３、１７００攻毒后，攻毒对照组死亡率为 ５０％，第 ３、４组分别为 ５％、１０％．结果表明，在有母源抗体水平的雏鸡中，１０ ｄ龄用 ＩＢＤＶ－Ａｂ复合苗免疫的效果优于商品疫苗，结果提示，ＩＢＤＶ高免抗体和中等毒力的ＩＢＤＶ疫苗接一定比例配合后。 The efficacy of a novel vaccine, infectious bursal disease virus-antibody complex vaccine (IBDV-Ab), was compared with that of a commercial IBDV vaccine in Shiqiza broiler with maternal antibodies against IBDV. Seventy-five birds were divided into four groups and raised in isolation units. Birds of group 3 and 4 were vaccinated at 10 days with IBDV-Ab vaccine and commercial vaccine, respectively, whereas chickens of group 1 and 2 were not vaccinated with any vaccine. The antibodies against IBDV were measured by enzyme-linked immunosorbent assay (ELISA) before and after vaccinations. All the broilers of groups 2, 3, 4 were challenged with a virulent IBDV strain at 35 days of age. The mean ELISA titres (ET) of group 3 and 4 at 21 days of age were 738 and 946, respectively, and these at 31 days of age were 2 403 and 1 700. After challenge, mortality of groups 2, 3 and 4 were 50% 1 5% and 10% , respectively. The results showed that the efficacy of vaccination with IBDV-Ab vaccine was better than that with commercial vaccine. The data herein suggested that being mixed with antibody against IBDV increase the efficacy of IBDV live vaccine.

关 键 词： 传染性法氏囊病 免疫 鸡 抗原抗体复合苗 疫苗

领　　域： [农业科学] [农业科学] [农业科学] [农业科学] [农业科学] [农业科学]