作　　者： ; ; (廖如燕）;

机构地区： 广东工业大学轻工化工学院

出　　处： 《食品研究与开发》 2012年第2期131-133,共3页

摘　　要： 建立乳及乳制品中致病性大肠杆菌(EPEC)的快检方法。以EPEC特异的微绒毛粘连基因(eae gene)和茵毛束形成编码基因(bfp gene)为模板,设计两对引物,荧光探针对EPEC进行特异性扩增。该方法快速,特异性好,对EPEC的最低检出量为1 cfu/μL(纯菌),检出时间为3 h,同一样品重复检测3次ct值的变异系数均小于5%。建立可快速特异检测乳及乳制品中EPEC的双重荧光PCR方法。 Establish a rapid method for detecting Enteropathogenic Escherichia coli（EPEC） in milk and milk product.Based on attaching and effacing lesion（eaeA gene） and bundle-forming pili（bfpA gene） of EPEC,two pairs of primers and Taqman-based fluorescent probe were designed.Then EPEC were detected by double fluorescent PCR assay.The method was rapid and specific.The limit of detection was 1 cfu/μL in 3 hours.Stability test showed that coefficient variables were all less than 5 %.A feasible,rapid,sensitive and specific double fluorescence PCR method was established to detect EPEC in milk an d milk product.

关 键 词： 两重荧光 乳及乳制品 致病性大肠杆菌

领　　域： [轻工技术与工程] [轻工技术与工程]