机构地区: 华南农业大学食品学院
出 处: 《食品科技》 2012年第2期233-238,共6页
摘 要: 以雨生红球藻粉为原料,选取乙酸乙酯:乙醇(1:1,v/v)为提取溶剂,以虾青素提取率为评价指标,在单因素实验的基础上,利用响应面法对雨生红球藻中虾青素的提取条件进行了优化。结果表明,影响虾青素提取条件的强弱分别为温度>液固比>时间,通过计算回归方程优化得到的最佳提取条件为:温度50℃,时间100 min,液固比1.5:1。通过与荆州公司提供的提取方法和碱提法对比,应用此方法虾青素提取率分别提高了44.31%和86.80%。 Haematococcus pluvialis powder was used as raw material, ethyl acetate and ethanol(volume ratio 1:1) was selected as the best extraction solvent. Based on the single factor experiment, the extraction rate of astaxanthin was taken as the evaluation index, response surface methodology was employed for optimizing extraction conditions of astaxanthin from Haematococcus pluvialis. The results showed that the effect of the extraction conditions was temperature 〉 radio of solution to material 〉 time, and the optimum conditions were: temperature 50 %, time 100 min, and radio of solution to material 1.5:1. Compared with the method provided by Jingzhou company and alkaline extraction method, it was proved that this improved method could increase the extraction rate of astaxanthin by 44.31% and 86.80%, respectively.