作　　者： ; ; ; ;

机构地区： 华南理工大学轻工与食品学院

出　　处： 《食品科技》 2012年第2期27-31,共5页

摘　　要： 探讨利用碱性蛋白酶酶解丝素蛋白制备ɑ-葡萄糖苷酶抑制肽的工艺条件。以丝素肽对ɑ-葡萄糖苷酶的体外抑制活性和水解度(DH)为主要评价指标,通过正交试验优化了ɑ-葡萄糖苷酶抑制肽的工艺条件。结果表明,在最佳工艺条件为温度60℃、反应pH8.5、底物质量浓度5%、加酶量为1200 U/g、反应时间100 min下测得丝素肽对ɑ-葡萄糖苷酶具有较高抑制率,为44.90%,此时DH为16.80%。其抑制活性与浓度有较大的依赖关系,而作用时间控制在40 min即可。此外,通过对丝素蛋白肽蔗糖和葡萄糖含量的检测,结果表明,尽管丝素肽中含有微量的葡萄糖,但抑制干扰作用甚微。 Silk fibroin was hydrolyzed by alcalase to produce the peptide with high a-glucosidase inhibition activity in vitro. The hydrolysis condictions were optimized according three indicators through orthogonal tests and the optimum reaction conditions were determined as follows： pH8.5, temperature 60℃, substrate concentration 5%, enzyme dosage 1200 U/g protein, and reaction time 100 min, under which the a-glucosidase-inhibition rate and DH of the product were 44.90% and 16.80%, respectively. The results shows that the a-glucosidase inhibition activity in vitro was related to the concentration of silk fibroin and the reaction time could be 40 min. Moreover, microamounts of glucose existing in silk fibroin, but they had less interrupting effect on the anti-hyperglycemic activity.

关 键 词： 碱性蛋白酶 丝素蛋白 葡萄糖苷酶 降血糖

领　　域： [轻工技术与工程] [轻工技术与工程]