作　　者： ; ; ; ; ;

机构地区： 淮阴工学院生命科学与化学工程学院江苏省凹土资源利用重点实验室

出　　处： 《中国酿造》 2012年第1期46-49,共4页

摘　　要： 目的:研究纤维素酶法辅助提取花生壳中木犀草素的最佳工艺条件。方法:采用HPLC法测定木犀草素含量,以提取率为指标,分别考察料液比、加酶量、pH值、温度、时间对纤维素酶酶解反应的影响,并将筛选工艺与目前文献的乙醇直接提工艺进行对比分析。结果:纤维素酶酶解花生壳的最佳工艺条件:料液比为1∶8(w/v)、酶用量为25U/g药材的量、酶解pH值为4.8、酶解温度50℃、酶解时间为6h。结论:与传统乙醇直接提取工艺相比,酶法辅助提取工艺提取花生壳中木犀草素的提取率提高了2.15倍。 Objective： The optimum conditions for cellulase-assisted extraction of luteolin from peanut hull were studied.Methods： The luteolin content was determined by HPLC.According to extraction rate,the effects of solid material-liquid ratio,enzyme concentration,pH value,temperature and reaction time on cellulase reaction were studied.Besides,the enzyme-assisted extraction was analyzed with the comparison of ethanol extraction technology.Results： The optimum reaction conditions of cellulase were determined as follows： solid material-liquid ratio 1：8（w/v）,25U cellulase added for 1g material,pH value 4.8,temperature 50℃ and reaction time 6h.Conclusion： Compared with the traditional ethanol extraction,the luteolin yield was improved by 2.15times.

关 键 词： 纤维素酶 提取 花生壳 木犀草素

领　　域： [理学] [理学]