作　　者： ; ; ; ; ; ;

机构地区： 深圳大学生命科学学院

出　　处： 《应用昆虫学报》 2012年第1期248-254,共7页

摘　　要： 本文研究东亚飞蝗Locusta migratoria manilensis(Meyen)雄性生殖系统组织解剖结构及三维可视化数字模型的构建。采用石蜡切片技术对东亚飞蝗进行组织切片,脱水、透明、HE染色和拍照;并应用冰冻切片技术将冰冻包埋剂包埋后的飞蝗雄性个体进行连续切片,进行截面图像信息采集,建立数据集;通过Photoshop、Image-Pro Plus(IPP)软件对雄性生殖器官截面图像进行分割、处理、序列化和三维重建。通过试验观察分析东亚飞蝗雄性生殖器官精巢、附腺、输精管、精球囊和射精囊及交配器的组织构造;并成功构建了飞蝗雄性生殖系统的三维结构可视化数字模型。该模型可以任意旋转,能从不同角度观察,该试验为研究和教学提供了理论基础。 We conducted a three-dimensional reconstruction of the genital system of male Locusta migratoria manilansis （Meyen）. The genital system was HE stained, and male adults embedded in frozen embedding medium （OCT） and sliced serially in accordance with the frozen section technique. Digital section image information was gathered and collated in a data set. Photoshop 7.0 and Image-Pro Plus （IPP） software were used to segment, process and merge all sectional images of genital organs and then to complete a three-dimensional reconstruction of the genital system. Using this method, the histological micro-structure of the testis, vas deferens, accessory gland, ejaculatory duct, seminal vesicle, ejaculatory sac and spermatophore sac were observed, and a three-dimensional visualized digital model of the genital system of L. m. manilansis’s was successfully constructed. The model can be rotated randomly and observed from different angles. These results provide the theoretical foundation for further study of the genital system of the male oriental migratory locust.

关 键 词： 东亚飞蝗 雄性生殖系统 石蜡切片 三维可视化

领　　域： [生物学]