作　　者： ; ; ; ; ; ;

机构地区： 石河子大学农学院

出　　处： 《生物技术》 2011年第5期48-50,共3页

摘　　要： 目的:分离出棉花抗枯萎病基因片段,并Blast分析其差异片段。方法:以抗枯萎病的棉花品种中棉12号为材料,采用苗期水培方法于三叶期进行枯萎病菌诱导处理,以不接菌为对照。用64对选择性扩增引物对诱导处理和对照的cDNA进行AFLP分析。结果:得到25个阳性差异片段,在GenBank中进行同源性序列比对,24个片段在GenBank数据库中发现同源序列,有1个功能未知。结论:作物的抗病机理复杂,涉及物质和能量代谢,信号传导,逆境响应及蛋白功能调控等方面。 Objective：To isolate a gene fragment of cotton resistance to Fusarium Wilt,and Blast analysis of the differential fragments.Method：In this study,resistance to Fusarium Wilt of cotton varieties in cotton No.12 for the material,the use of seedlings in water culture method for Fusarium-induced leaf stage treatment to the control without inoculation.With 64 pairs of selective primers for induction treatment and control of cDNA AFLP analysis.Result：The positive difference between 25 fragments in the GenBank sequence homology comparison of the 24 fragments found in the GenBank database,homology,there is an unknown function.Conclusion：To further clarify the cotton molecular mechanisms of resistance to Fusarium Wilt provide a reference for the use of biotech cotton varieties against wilt lay a certain foundation.

关 键 词： 棉花 枯萎病 差异表达基因

领　　域： [农业科学] [农业科学]