作　　者： ; ; ; ;

机构地区： 西北农林科技大学园艺学院农业部西北园艺植物种质资源与遗传改良重点开放实验室

出　　处： 《西北农业学报》 2011年第10期74-78,共5页

摘　　要： 以大白菜纯合自交系92S105为试材,对激素、外植体类型、苗龄等再生因素进行优化,研究大白菜再生芽对抑菌抗生素及其筛选抗生素的敏感性。结果显示,在MS附加0.3 mg.L-1TDZ和0.5 mg.L-1NAA的再生培养基上,92S105再生率最高,可达到76.19%。生根培养基为1/2MS附加1.0 mg.L-1IBA或1.0 mg.L-1IAA,生根率都达到100%。在遗传转化中,以5 mg.L-1潮霉素作为筛选抗生素,来筛选抗性芽和抗性植株。用头孢噻肟钠和羧苄青霉素作为抑菌剂,均抑制不定芽的分化,但羧苄青霉素对不定芽分化影响较小,所以使用羧苄青霉素作为抑菌剂。 The effects of hormone,explant types and seedling stage on regeneration rate of adventitious bud were studied in this experiment with a homozygous inbred lines 92S105 of Chinese cabbage.And the sensitivity of regeneration shoot to antibiotics were examined.The results showed that the maximum shoot regeneration frequency,76.19%,was obtained in Murashige and Skoog medium supplemented with 0.3 mg·L-1TDZ and 0.5 mg·L-1NAA.The rooting medium is 1/2 MS supplemented with 1.0 mg·L-1IBA or IAA,the root frequency both can reach 100%.In the transformation experiment,5 mg·L-1Hyg can be used to select the putative regeneration shoots and plants.The data showed that the two bacteriostats,cefotaxime and carbencillin,both inhibit adventitious bud differentiation,but carbencillin is better than cefotaxime.

关 键 词： 大白菜 再生体系 抑菌剂 潮霉素

领　　域： [农业科学] [农业科学]