作　　者： ; ; ; ; ; ; ;

机构地区： 东北林业大学

出　　处： 《中国畜牧兽医》 2012年第1期64-67,共4页

摘　　要： 本研究扩增肝片吸虫(Fasciola hepatica,Fh)谷胱甘肽硫转移酶(GST)基因,并进行同源性分析。根据GenBank发表的部分Fh GST基因序列设计并合成一对特异性引物,利用RT-PCR方法扩增出Fh GST基因完整的开放阅读框(openreading frame,ORF),测定序列,使用分子生物学软件进行同源性分析。获得Fh GST基因全长682bp,编码218个氨基酸,与澳大利亚分离的肝片吸虫GST同源性较高,与大片吸虫和卫氏并殖吸虫的GST也有较高的同源性。不同虫株GST基因具有较高的同源性,因此Fh GST蛋白不适合用作诊断抗原,但由于其存在交叉反应,GST基因作为分子疫苗的候选基因具有重要意义。肝片吸虫GST基因的克隆,为进一步研究GST蛋白的功能和作用奠定了基础。 The glutathione S-transferase gene of Fasciola hepatica was amplified and its homology was analyzed.According to the published genomic sequence of Fh GST in GenBank,a pair of primers was designed and the open reading frame of GST gene of Fasciola hepatica was amplified by RT-PCR.The homology of GST gene was analyzed by molecular biological soft.The PCR analysis results showed that the Fh GST gene was 682 bp,and encoded 218 amino acid.It indicated that the Fh GST gene was highly homologous with Fasciola hepatica isolated from Australia,Fasciola gigantica and Paragonimus westermani.Because of the high homology of the GST gene of different parasite,it could not be used in diagnosis assay with Fh GST protein,but could be used in gene vaccine.The clone of Fh GST gene had advantages to investigate the function and effect of GST protein.

关 键 词： 肝片吸虫 基因 序列分析

领　　域： [生物学]