作　　者： ; ; ; ; ;

机构地区： 中华人民共和国农业部

出　　处： 《中国兽医科学》 2012年第1期13-18,共6页

摘　　要： 为研究MHC B-L基因的多态性与鸡白痢沙门氏菌感染之间的关系,采用PCR产物直接测序技术对供试鸡MHC B-L基因外显子2进行单核苷酸多态性检测,分别利用Plink1.07软件和PHASE V2.1软件进行病例-对照关联分析和单倍型分析。结果发现,在270bp的序列内共检测到42个单核苷酸多态性位点(SNPs)。C.280C>A位点等位基因在病例-对照样本中的分布存在显著差异(P<0.05);C.246G>T、C.249A>G位点基因型在病例-对照样本中的分布存在显著差异(P<0.05)。C.229T>A和C.249A>G位点组成的单倍型与抗鸡白痢性状差异不显著(P>0.05);同时C.285G>A、C.287A>T和C.288C>A位点组成的单倍型与抗鸡白痢性状的差异也不显著(P>0.05)。结果表明,MHC B-L基因外显子2多态性与抗鸡白痢性状显著相关,为开展沙门氏菌抗性分子标记辅助选择研究提供了一定的依据。 To study the association between MHC B-L gene polymorphism and Salmonella pullorum infection,polymerase chain reaction sequence-based typing method was used to detect the polymorphism of MHC B-L gene exon 2. The Plinkl. 07 and PHASE V2. 1 software was used as case-control association study and haplotype analysis, respectively. There were 42 single nucleotide polymorphisms（SNPs） in 270 bp DNA sequence. The distribution of C. 280C〉A alleles had a significant difference in case-control samples. The distribution of C. 246G〉T and C. 249A〉G genotypes had a significant difference in case-control samples. Haplotypes within the C. 229T〉A and C. 249A〉G sites and within the C. 285G〉A,C. 287A〉T and C. 288C〉A sites did not associate with anti-pullorum disease trait. The results showed that the polymorphism of MHC B-L gene exon 2 was significant associated with anti-pullorum disease trait, and provided some bases for developing marker assisted selection programmes for anti-salmonellosis.

关 键 词： 鸡白痢 易感性 单核苷酸多态性位点 单倍型

领　　域： [农业科学] [农业科学] [农业科学]