作　　者： ; ; ; ; ; ;

机构地区： 广东省农业科学院

出　　处： 《昆虫学报》 2011年第12期1335-1340,共6页

摘　　要： 昆虫变态发育过程中,蜕皮激素通过一系列的激素相关转录因子进行信号的转导和放大,从而完成对生长变态发育的调控,其中蜕皮激素受体(EcR)及转录因子BR-C和E74A可能作为早期因子发挥作用。为了研究这3个早期转录因子在鳞翅目昆虫中的功能,本研究采用体外合成dsRNA的方法,将合成的dsRNA分别注射熟蚕期的家蚕Bombyx mori,进行RNA干扰,并对这3个基因被RNA干扰后的形态变化和相关的转录因子基因BmHR3A,BmHR39,BmβFTZ-F1,BmE74B和BmE75B的表达进行了实时荧光定量PCR检测。形态学观察结果显示,这3个基因被RNAi后出现异常的蛹和蛾、小翅和死蛹等表型。qPCR检测结果表明:RNAi BmEcR后导致了BmE74A,BmE74B,BmHR3A和BmβFTZ-F1表达水平的显著降低(P<0.05),RNAi BmE74A后导致了BmEcR,BmBR-C,BmE74B,BmHR3A和BmβFTZ-F1表达水平的显著降低(P<0.01),而RNAi BmBR-C后则导致所有被检测的蜕皮激素受体及其相关转录因子的mRNA水平都显著降低(P<0.01)。据此推测,BmBR-C可能在蜕皮激素信号的早期发挥作用,并对其他相关转录因子和蜕皮激素受体有调控作用。 In insects,ecdysone signal transduction pathway is mediated by a cascade of transcription factors,and ecdysone receptor（EcR）,Broad complex（BR-C） and E74A are believed to be the early response factors.To study the functions of these early transcription factors in Lepidoptera,doublestranded RNAs（dsRNAs） of these genes of Bombyx mori were synthesized in vitro and injected into the wandering larvae.Phenotype changes were observed and the expression of these and related genes were analyzed by quantitative real-time PCR（qPCR）.Asynchronous pupation and eclosion,deviant wing and death of pupae and moths were observed after interference of BmEcR,BmBR-C and BmE74A.The qPCR results revealed that partial silence of BmEcR decreased the mRNA levels of BmE74A,BmE74B,BmHR3A and BmβFTZ-F1.Partial silence of BmE74A reduced the mRNA levels of BmEcR,BmBR-C,BmE74B,BmHR3A and BmβFTZ-F1,while partial silence of BmBR-C resulted in a significant decrease in the expression levels of all tested genes except for BmIAP.These results implied that BmBR-C may act at the earlier stage in ecdysone signal pathway and regulate the expression of other transcription factor genes as well as ecdysone receptor gene BmEcR in the silkworm.

关 键 词： 家蚕 干扰 变态 蜕皮激素 转录因子

领　　域： [生物学]