作　　者： ; ;

机构地区： 中国科学院南海海洋研究所

出　　处： 《热带海洋》 2000年第1期58-63,共6页

摘　　要： 调查了饵料维生素C对青石斑鱼Epinephelusawoara非特异性的体液和细胞免疫调节作用。在冰冻饵料小杂鱼中添加不同剂量的维生素C(每公斤饵料鱼含量分别为 0 ,50 0 ,1 0 0 0 ,1 50 0和 2 0 0 0mg) ,连续投喂青石斑鱼 2 0周后 ,青石斑鱼血液白细胞总数及不同种类白细胞 (包括淋巴细胞、中性粒细胞和单核细胞 )组成比例不受饵料维生素C的影响。青石斑鱼血清补体经典型溶解羊红细胞的能力随饵料维生素C添加量的提高而显著增强 (p<0 .0 1 )。然而 ,维生素C对血清的杀菌活性没有作用。鱼体非特异性的细胞免疫活动 (包括血液白细胞和头肾细胞的吞噬活动 ) The non specific humoral and cellular immunomodulatory effects of dietary vitamin C(Vc) on grouper Epinephelus awoara were studied.Grouper were provided with frozen diet fish(trash fish)supplemented with different doses of vitamin C(0,500,1?000,1?500,2?000?mg·kg -1 )continuously for 20 weeks.It was found that the total blood leucocyte number and the composition proportion of different leucocytes including lymphocyte,neutrophil and monocyte were unaffected by vitamin C.The activity of classical haemolysis of serum complement to sheep red blood cells(SRBC)significantly increased with the increase of vitamin C( p <0.01).However,no difference was seen in the bactericidal complement activity of serum among fish treated with dietary vitamin C.The non specific cellular defence mechanisms were examined.Blood leucocyte phagocytosis to Staphylococci aureus and phagocytic activity of adherent head kidney cells were unaffected by dietary vitamin C.

关 键 词： 青石斑鱼 维生素 免疫刺激剂 非特异性免疫

领　　域： [生物学] [农业科学] [农业科学]