作　　者： ; ; ; ; ; ; (黄科林）; (孙果宋）;

机构地区： 广西大学化学化工学院

出　　处： 《食品科学》 2011年第23期186-191,共6页

摘　　要： 以蚕蛹蛋白为原料,使用碱性蛋白酶、木瓜蛋白酶、胰蛋白酶、菠萝蛋白酶对其进行酶解,采用1,1-一苯基-2-三硝基苯肼自由基(DPPH自由基)法、超氧阴离子自由基(O-2.)法、羟自由基(.OH)法和血压紧张素转化酶(ACE)法对酶解产物进行抗氧化和降血压活性分析,筛选同时具有较强抗氧化和降血压活性的酶解产物,并确定获得较强活性时的水解度,再通过响应面对该水解度下酶解工艺条件进行优化。结果表明:碱性蛋白酶在水解度为20%左右时获得的水解产物抗氧化和降血压效果较好,并由响应面分析结果表明酶解温度和加酶量之间存在显著的交互作用,同时得到最佳酶解工艺条件为:酶解温度51℃,pH值为9.05,加酶量为0.38%,酶解时间3h,在此条件下,水解度为20.53%,再通过实验验证其误差值小于5%,说明采用响应面试验设计方法对蚕蛹蛋白酶解体系进行优化具有较好的可靠性。此时酶解产物的DPPH自由基抑制率为51.2%,ACE抑制活性为50.3%。 In the present study,silkworm pupae protein hydrolysates at different degree of hydrolysis were prepared with alcalase,papain,trypsin,and bromelain,respectively and their scavenging activity against DPPH,superoxide anion and hydroxyl free radicals and antihypertensive activity were comparatively determined,with the result that hydrolyzing silkworm pupae protein by alcalase to a degree of hydrolysis（DH） of 20% yielded a hydrolysate with better antixodiant and antihypertensive activities.Three main process conditions for the hydrolysis of silkworm pupae protein by alcalase,including temperature,pH and enzyme dosage were further optimized using response surface methodology.The results of response surface analysis indicated that temperature and enzyme dosage had a significant interactive effect on the hydrolysis of silkworm pupae protein and that the optimal hydrolysis conditions for achieving 20% DH and minimizing costs were 51.0 ℃,pH 9.05 and 0.38% enzyme/substrate ratio.Under these conditions,the experimental DH was 20.53%,with an error of less than 5% as compared with the predictive value,and the DPPH radical scavenging rate and ACE inhibitory rate of the obtained hydrolysate were 51.2% and 50.3%,respectively.

关 键 词： 蚕蛹蛋白 抗氧化肽 降血压肽 响应面 水解度

领　　域： [生物学]