机构地区: 华东理工大学生物工程学院生物工程系
出 处: 《华东理工大学学报(自然科学版)》 1999年第6期563-566,共4页
摘 要: 用Dextran T2000,经环氧氯丙烷交联并氧化产生部分羧基,偶联对氨基苯甲脒制得水溶性亲和载体巨配基。配基密度为71.4μm ol/g。截留分子量为10 万的超滤膜对所制亲和载体的截留率大于99.5% ,但尿激酶能自由通过。将比活为500IU/A280的尿激酶粗品经亲和超滤得到纯化的尿激酶,比活达66 300IU/A280,纯度提高133 倍,回收率达83.4% 。 A water soluble urokinase specific macroligand was prepared by oxidizing and cross linking Dextran T2000 simultaneously with epichlorohydrin. The pendant carboxyl groups formed were then coupled with p aminobenzamidine. The ligand density of the macroligand was 71.4μmol/g dextran. A hollow fiber ultrafilter with 100 000 MWCO nominal (molecular weight cut off) membrane showed a rejection over 99.5% for the macroligand, but no retention for free urokinase by means of batchwise affinity ultrafitration the starting crude urokinase with specific activity of 500IU/ A 280 was purified with a yield of 83.4% and a purification factor of 132.