作　　者： ; ; ; ; ; ; ;

机构地区： 佛山科学技术学院

出　　处： 《食品科学》 2011年第21期156-160,共5页

摘　　要： 研究花色苷矢车菊素-3-O-β-D-葡萄糖苷(cyanidin-3-O-β-D-glucoside,C3G)及其苷元矢车菊素(cyanidin)对代谢综合征紧密相关的一系列核受体转录活性的影响。将维甲酸X受体(RXR)、孕烷X受体(PXR)、雌激素受体(ER)、法尼醇X受体(FXR)、肝脏X受体α和β(LXRα,LXRβ)、3种过氧化物酶体增殖剂激活受体(PPARα、PPARγ、PPARδ)等核受体-萤光素酶报告基因和绿色荧光蛋白基因内参转染至人肝细胞LO2建立模型,分别以1、10、100μmol/L的Cyanidin或C3G干预培养细胞24h,筛选可被Cyanidin或C3G激活的核受体类型。结果显示,与溶剂对照组(0.1%DMSO)相比,3种浓度的Cyanidin对RXR都有抑制作用,但是仅在100μmol/L浓度条件下对PXR有较弱的激活作用(1.42±0.19,P<0.05);C3G则对PXR、ER、LXRα、LXRβ、PPARγ、PPARδ等多种核受体有激活作用(P<0.05),其中对PXR、LXRα、LXRβ、PPARγ等核受体的激活倍数超过对照组50%以上。实验结果提示,花色苷改善代谢综合征的作用机制可能与它对一系列细胞核受体的激活作用有关;比较Cyanidin和C3G对核受体的激活能力,发现C3G的作用效果强于其苷元Cyanidin。 The effects of anthocyanin [cyanidin-3-O-β-D-glucoside（C3G）] and its aglycone [cyanidin] on transcriptional activity of nuclear receptors closely related to metabolic syndrome were investigated.Human liver LO2 cells were transfected with several plasmids including retinoid X receptor（RXR）,pregnane X receptor（PXR）,estrogen receptor（ER）,farnesol X receptor（FXR）,liver X receptors（LXRα and LXRβ）,and peroxisome proliferator activated receptors（PPARα,PPARγ and PPARδ）,respectively.Plasmids with the nuclear receptor element luciferase gene,and green fluorescent protein were constructed as nuclear receptor reporter models.The transfected cells were cultured with media containing cyanidin or C3G at the concentrations of 1,10 μmol/L and 100 μmol/L,respectively for 24 h.The results indicated that RXR was inhibited by cyaniding at all concentrations tested,and only PXR model was slightly stimulated by cyanidin at the concentration of 100 μmol/L（1.42 ± 0.19 fold,P 0.05 compared with 0.1% DMSO as the control）.However,other nuclear receptors including PXR,ER,LXRα,LXRβ,PPARγ and PPARδ could be significantly stimulated by C3G（P 0.05）,and the transcriptional activity of PXR,LXRα,LXRβ and PPARγ could be increased by over 50% when compared to 0.1% DMSO.Therefore,the mechanism of anthocyanidin improving metabolic syndrome was likely to be correlated with its augmentative effect on transcriptional activity of nuclear receptors,and it could be concluded that C3G was generally more effective than its aglycone cyanidin.

关 键 词： 矢车菊素 葡萄糖苷 核受体 萤光素酶报告基因 转录活性

领　　域： [轻工技术与工程] [轻工技术与工程]