作　　者： ; ; ;

机构地区： 华南理工大学生物科学与工程学院

出　　处： 《微生物学报》 2011年第12期1669-1674,共6页

摘　　要： 【目的】探讨黄曲霉毒素对一种发光细菌——费氏弧菌发光的抑制效应。【方法】黄曲霉毒素或产黄曲霉毒素的菌株培养液对费氏弧菌进行处理后,利用多功能酶标仪检测费氏弧菌的发光强度,研究黄曲霉毒素对费氏弧菌发光的影响。【结果】黄曲霉毒素浓度的对数值与费氏弧菌发光的抑制率呈线性关系,依据所得的回归方程可以快速准确地检测不同微生物产毒素的情况:6株不同来源的黄曲霉菌株均能够产毒素,以黄曲霉毒素含量表示的毒素量在14.94-46.45mg/L之间,1株米曲霉不产毒素。【结论】费氏弧菌发光强度的改变可以较准确地反映微生物产毒素的能力,尤其是微生物产黄曲霉毒素的能力,为在工农业生产中快速检测黄曲霉毒素提供了新的线索,有望发展成为一种检测黄曲霉毒素的新技术。 [Objective] In the present study,we aim to evaluate the inhibitory effect of aflatoxin on Vibrio fischeri luminescence.[Methods] V.fischeri culture is treated with aflatoxin or the culture broth of aflatoxin-producing strains,and the luminescence intensity of V.fischeri is detected to analyze the influence of aflatoxin on V.fischeri.[Results] The logarithmic value of aflatoxin concentration and the decrease ratio of V.fischeri luminescence is in a linear relationship.Based on the regression equation between aflatoxin concentration and luminescence decrease of V.fischeri,the toxin-producing status of different microbes can be detected quickly and exactly： all of six tested Aspergillus flavus strains show toxigenicity to V.fischeri,and their toxin yield reached 14.94 mg/L-46.45 mg/L（represented by aflatoxin concentration）,while the tested Aspergillus oryzae shows no toxigenicity.[Conclusion] The above data showed that the luminescence change of V.fischeri could exactly reflect the capability of various microbes to produce toxin（especially aflatoxin）,which provided a new clue for rapid detection of aflatoxin in industrial and agricultural production and could be developed as a potential method for aflatoxin assay.

关 键 词： 黄曲霉毒素 费氏弧菌 光抑制 生物毒素

领　　域： [生物学]