作　　者： ; ; ; ; ; ; ; ;

机构地区： 中国水产科学研究院南海水产研究所

出　　处： 《食品工业科技》 2011年第12期455-457,461,共4页

摘　　要： 建立了高效液相色谱测定水产品中呋喃西林残留量的方法。通过对比不同有机溶剂的提取效果,表明使用单溶剂提取回收率均不高,采用丙酮和二氯甲烷的混合溶液提取效果更好,其中比例为3∶7时最佳。比较了振荡法、超声波法和超声波加热法等的提取能力,结果表明,超声波加热法提取效果最好。以丙酮-二氯甲烷作为提取剂、超声波加热(35kHz,强度100%,40℃)进行提取,提取时间15min、提取2次,提取率可达90%以上,方法回收率在75%～100%之间,相对标准偏差小于10%,检测限能达到0.5μg/kg。本方法操作简便,回收率较高,稳定性好。 A High-performance liquid chromatography（HPLC）method for the nitrofuran residues in aquatic products was proposed. Extracted by methylene chloride,acetone,methanol,acetonitrile and ethyl acetate respectively,the recovery rate of nitrofuran cannot meet the requirement. Better result could be acquired by extracting with a mixed solution of acetone and dichloromethane,while the ratio was 3∶ 7. Ultrasonication assisted by heating was the best method for facilitating the extraction,comparing with oscillation,ultrasonication methods. Extracted with Acetone-dichloromethan agent,ultrasonication assisted by heating（35kHz,intensity 100%,40℃）,treated twice with 15min each time,the nitrofuran can be extracted well,the extraction rate was higher than 90%. A method,of simplicity,high recovery and good stability,which the recovery was 75%～100%,the relative standard deviation was less than 10%,and the detection limit can reach 0. 5μg/kg was attained.

关 键 词： 呋喃西林 超声波提取 高效液相色谱

领　　域： [轻工技术与工程] [轻工技术与工程]