作　　者： ; ; ; ;

机构地区： 暨南大学

出　　处： 《食品工业科技》 2011年第12期261-264,396,共5页

摘　　要： 以豆皮为原料,用黑曲霉ATCC16404固体发酵产木聚糖酶,采用DNS测定酶活,研究了最佳产酶培养基配比,优化产酶最佳条件。单因素实验结果表明,最佳培养基配方为:以硫酸铵为氮源,矿质元素添加硫酸镁,添加量均为2%(w/w以豆皮干重计),豆皮颗粒大小为25～50目,不外加碳源,不加表面活性剂。选择料液比、初始pH、培养时间、接重量优化产酶条件,正交实验结果表明,最佳产酶条件为:料液比1∶1.00(w/v)、初始pH5.5,培养时间3d,1.0×107/mL浓度孢子悬浮液接种0.3mL/10g,在此条件下酶活为366.29U/g。 Components of incubation media and followed by the best conditions for enzyme production were optimized in solid-state fermentation of soybean hull by Aspergillus niger ATCC16404 to produce xylanase,whose activity was determined by DNS. Results of single factor experiments showed that the optimum components of incubation media contain ammonium sulfate and magnesium sulfate used as nitrogen source and mineral element,with 2% addition based on the weight of 25～50 mesh dry soybean hull,and without additional carbon source and surfactant. Solid-to-liquid ratios,initial pH,incubation time,inoculum size were selected to optimize the condition of enzyme production. Orthogonal experiment showed the highest xylanase activity was 366. 29U/g at the optimum fermentation conditions of solid-to-liquid ratio 1∶ 1. 00,initial pH 5. 5,3d incubation,0. 3mL /10 g spore suspension of 1. 0×107/mL concentration.

关 键 词： 黑曲霉 豆皮 木聚糖酶 固体发酵

领　　域： [轻工技术与工程] [轻工技术与工程]