作　　者： ; ; ; ; ;

机构地区： 暨南大学生命科学技术学院生物矿化与结石病防治研究所

出　　处： 《无机化学学报》 2011年第12期2425-2430,共6页

摘　　要： 采用纳米粒度仪和透射电子显微镜(TEM)比较研究了10例尿石症患者和10例健康对照者的尿液经不同孔径滤膜(0.22、0.45、1.2、3.0、10μm)过滤后,尿微晶平均粒径(d)、粒度分布、自相关曲线、ζ电位和聚集状态的变化。随着滤膜孔径由0.22μm增至10μm,患者尿微晶的d值由162 nm增至3 227 nm,自相关曲线平滑性变差,自相关时间(Ta)由1.92 ms增至2100 ms,ζ由-2.65 mV减小至-9.21 mV,TEM显示尿微晶尺寸差异大,部分尿微晶处于聚集状态。而对照者尿微晶的d值仅由187 nm增加至1 010 nm,自相关曲线平滑,Ta由1.40 ms增加至6.67 ms,ζ平均值由-5.22 mV减小至-6.89 mV,TEM显示尿微晶均匀分散,尺寸较小。上述结果表明:尿石患者的尿液体系不稳定,其尿微晶聚集程度高,导致尿石症形成的危险性增加。 By means of nanoparticle size analyzer and transmission electron microscopy（TEM）,the effect of membrane pore size on the properties of crystallites in urine of 10 lithogenic patients and 10 healthy subjects were comparatively investigated after urines were filtrated through microporous membrane with a size of 0.22,0.45,1.2,3.0,10 μm,respectively.These properties include average particle size（d）,size distribution,intensity-autocorrelation curve,ζ potential and aggregation state.With the increase of pore size from 0.22 μm to 10 μm,d value of the urinary crystallites in urine of lithogenic patients increased from 162 nm to 3 227 nm,the autocorrelation time（Ta） increased from 1.92 ms to 2 100 ms,the average value of ζ decreased from-2.65 mV to-9.21 mV,respectively;and the autocorrelation curves were of fluctuating and unsmooth,TEM showed that the size difference of the crystallites was large and some crystallites were in the aggregation state.However,for the crystallites in urine of healthy subjects,d increased only from 187 nm to 1010 nm,Ta increased from 1.40 ms to 6.67 ms,the average value of ζ decreased from-5.22 mV to-6.89 mV,the autocorrelation curves were of regular and smooth,TEM showed that the crystallites were well-dispersed with a smaller size.The above results demonstrated that the urinary system of lithogenic patients is unstable and the aggregation degree of their urinary ctystalllites is high.So the risk of urolithiasis formation of urinary crystallites of patients is larger than that of healthy subjects.

关 键 词： 纳米微晶 电位 自相关曲线 聚集

领　　域： [理学] [理学]