作　　者： ; ; ; ; ; ;

机构地区： 西南大学动物科技学院

出　　处： 《生态环境学报》 2011年第10期1496-1502,共7页

摘　　要： 研究了硫丹暴露对草鱼肝脏、肌肉抗氧化酶及脑乙酰胆碱酯酶活性的影响。结果表明,硫丹24 h暴露可诱导草鱼脑AChE活性,当暴露时间延长或质量浓度升高时,AChE活性表现为受抑制,120 h较高质量浓度组抑制率为41.8%和56.2%,抑制率与暴露时间呈良好的线性相关。硫丹暴露24 h后,草鱼肝脏及肌肉SOD、GSH-Px活性受到显著影响,表现出先诱导后缓慢降低的趋势,120 h后SOD活性显著低于对照组水平,GSH-Px活性与对照组无显著差异。在抗氧化酶受到影响的同时,鱼体脂质过氧化LPO程度不断上升,组织MDA含量逐渐增大,96 h达到最高值。硫丹可影响草鱼AChE及抗氧化酶活性,其变化可作为生物标志物,来评价化学污染物对水生动物的生物学效应。 The effects of endosulfan exposure on the induction of oxidative stress and the alteration of AChE activities were studied in liver,muscle and brain samples from Ctenopharyngodon idellus.The results showed that the AChE activities of brain in Ctenopharyngodon idellus was stimulated after 24 h exposure.However,The activities of AChE were restrained when the exposure time and concentration was increased,and the inhibition rate was 41.8% and 56.2% in higher concentration groups after 120 h exposure,it showed a good linear correlation between the inhibition rate and the exposure time.The activities of SOD and GSH-Px in liver and muscle of Ctenopharyngodon idellus were significantly affected after 24 h exposure,showing a slow decrease after induction,then the SOD activities was significantly lower than the controls level,while the GSH-Px activities with no significant differences between the controls.The LPO level was rising when the antioxidant enzymes are affected in the same time,the MDA contents were increased,and reached the highest value after 96 h exposure.In conclusion,endosulfan impacts AChE and antioxidant enzyme activities on Ctenopharyngodon idellus,the adverse effects are sensitive parameters to use as the biomarker to assess the chemical pollutants on the biological effects of aquatic animals.

关 键 词： 硫丹 草鱼 乙酰胆碱酯酶 抗氧化酶

领　　域： [环境科学与工程]