作　　者： ; ; ;

机构地区： 华南农业大学食品学院广东省高等学校食品质量安全重点实验室

出　　处： 《安徽农业科学》 2011年第32期19843-19844,共2页

摘　　要： [目的]合成并鉴定有机磷农药三唑磷人工抗原。[方法]采用活泼酯法将三唑磷半抗原(TZPM-Hap)与牛血清蛋白(BSA)和卵血清蛋白(OVA)偶联,制备出免疫抗原(TZPM-A-BSA)和包被抗原(TZPM-A-OVA),并通过紫外扫描和动物免疫试验对其进行了鉴定。[结果]紫外扫描和动物免疫试验结果表明人工抗原合成成功。[结论]为制备单抗及开发快速、简便的三唑磷ELISA试剂盒奠定了基础。 [Objective] The aim was to synthesize and identify an organophosphate insceticide triazophos artificial antigen.[Method] Artificial antigens for organophosphate insecticide triazophos including one immunogen and one coating antigen were prepared.The hapten（TZPM-Hap） of triazophos was coupled to bovine serum albumin（BSA） by the active ester method to prepare the artificial immunogen TZPM-A-BSA.Coupling of TZPM-Hap to ovalbumin（OVA） by the active ester method produced the coating antigen TZPM-A-OVA.The artificial antigen was identified by ultraviolet scanning and immunizing animal experiment.[Result] Immunogen and coating antigen were prepared successfully and confirmed by ultraviolet spectrometry and immunizing animal.[Conclusion] The research lays a basis for the preparation of monoclonal antibody and the development of quick and simple triazophos ELISA kits.

关 键 词： 三唑磷 活泼酯法 人工抗原

领　　域： [农业科学] [农业科学]