机构地区: 中国农业科学院北京畜牧兽医研究所
出 处: 《中国动物营养学报》 1993年第1期7-14,共8页
摘 要: 大肠中微生物对蛋白质的降解与合成作用是用全收粪法测定饲料氨基酸生物学效价的主要干扰因素。利用回——直肠吻合术。游离大肠,制作专用于评定氨基酸生物学效价的荷术猪(Hostal swine)不仅可以避免大肠中微生物的作用,而且可以简化用瘘管法收集回肠末端排泄物的采样程序,减少误差来源,提高测值可信度。但采用这种方法时,如何取得有代表性的排泄物样品仍是保证测值精确度的关键问题。为此本试验研究了饲喂粗蛋白质量分别为9.6%、13.5%、17.0%和20.6%的四种半纯合日粮时,荷术猪每天的回肠末端干物质排泄量和排泄物中氨基酸含量的变化规律。以期为确立测定氨基酸生物学效价的方案提供科学依据。结果指出:①喂给不同半纯合日粮时,荷术猪回肠末端干物质排泄量的变异系数(C.V%)随日粮干物质表观消化率(ADDM)的增加而增加;②从喂给不同日粮的荷术猪回肠末端干物质排泄量的变化规律看,如以每3头试验猪统计,预饲3天后,收集4天排泄物可保证排泄量测值的准确性;③排泄物样品中氨基酸含量的稳定性并不因半纯合日粮饲喂期的延长而改善,因此测定氨基酸表观消化率时,可以根据干物质排泄量的稳定性来确定适宜的排泄物收集期。 The action of microbes on digesta protein in the large intestine is the main disturbing factor in determing amino acid availability with swine. Using the hostal animil with ileo-rectal anastomosis can avoid the microbiotic effect in the large intestine, also simplify the sampling procedure of excreta than using swine with ileo-cannula. Nevetheless how to take representive sample is still a important problem. This test studied the variation of the excreta of ileo-rectal anastomosised swine feeding 4 semi-purified diets containing 9.6%, 13.5%, 17.6% ant 20. 6% crude protein, respectively. The result showed: (1) the higher the apparent digestibility of dry matter of the diet, the larger the coefficient of varation(C. V%) of the quantity of dry matter excreta each day; (2) the content of animo acids in excreta is affected by the length of feeding period of semi—purified diets; (3) accroding to the varation of the quality of dry matter of excrata, a four day's period collection of excreta is proper for meeting the requirement of precision for sampling excreta.
领 域: [农业科学]