作　　者： ; ; ; ; ;

机构地区： 广东省农业科学院

出　　处： 《生物技术通报》 2008年第S1期200-209,共10页

摘　　要： 根据植物NBS类抗病基因保守氨基酸序列P-loop和疏水氨基酸GLPL保守序列设计简并引物,从香蕉抗镰刀菌枯萎病(4号小种)材料GCTCV-119的基因组DNA及cDNA中扩增获得9个DNA片段和10条cDNA片段,均编码为通读的氨基酸序列,命名为"BR-1"-"BR-19",GenBank登录号依次为EF515833-EF515836, EU123871-EU123885。同源性分析表明,均与已报道的植物抗病基因有不同程度的同源性,具有P-loop(Kinase-1a)、Kinase-2、RNBS-B(Kinase-3a)以及GLPL等保守氨基酸序列,属于non-TIR-NBS类候选抗病基因。其中,BR-5和BR-6与番茄抗镰刀菌枯萎病番茄专化型I2、I2-1和I2-2基因聚为一类,可能与香蕉镰刀菌枯萎病的抗性相关。 According to the NBS class conservative regions of plant resistant gene,the degenerate primers were designed by P-loop and hydrophobic amino acid conservative region. RGAs including 9 DNA fragments and 10 cDNA fragments were obtained from the germplasm GCTCV-119,which is resistance to fusarium wilt.Analysis of the deduced amino acids of these RGAs showed that their domain structures were NBARC and belonged to NBS-class resistance gene candidates,containing 4 conservative amino acid domains of P-loop(Kinase -1a),Kinase-2,RNBS-B(Kinase-3a)and hydrophobic amino acids conservative rgeion(GLPLALKVL).These 19 fragments, designated as BR-1,BR-2,to BR-19,have been deposited in GenBank with accession numbers EF515833- EF515836,EU123871-EU123885.BR-5 and BR-6 were clustered a group with I2,I2-I and I2-2 gene of tomato resistance to fusarium wilt,and may be related with banana resistance fusarium wilt.

关 键 词： 香蕉 抗病种质 抗病基因类似物 核苷酸结合位点

领　　域： [农业科学] [农业科学]