作　　者： ; ; ; ; ;

机构地区： 佛山科学技术学院生命科学学院广东省高校预防兽医学重点实验室

出　　处： 《畜牧与兽医》 2011年第11期21-24,共4页

摘　　要： 以胸膜肺炎放线杆菌1型参考菌株App-259株基因组DNA为模板,PCR扩增apxIA全基因,构建表达重组质粒apxIA-c2X,并将其转化至大肠杆菌TB1,SDS-PAGE和Western-blot分析表达产物。结果表明:成功构建了apxIA-C2x表达质粒,重组菌在15℃经0.2 mmol/L IPTG诱导16 h获得高效表达,融合蛋白大小为150 ku左右,目的蛋白约50%可溶,可溶蛋白经Amylose树脂亲和柱纯化,纯度可达到95%。本研究为猪传染性胸膜肺炎诊断抗原和亚单位疫苗的研制奠定了基础。 The 3069bp fragment of apxIA was amplified from the reference strain 259 of Actinobacillus pleuropneumoniae by PCR and cloned into the pMAL-c2X vector. The recombinant expression vector was transformed into Escherichia coli TB1 and induced by IPTG for expression. The target protein was purified by Amylose Column. The results showed that the recombinant expression plasmid apxIA-c2X was successfully constructed. The SDS-PAGE and Western-blot analysis showed that the apxIA gene could be expressed efficiently in E. coli TB1 with the induction of 0. 2mmol/L IPTG for 16 hours at 15℃. Molecular weight of the fusion protein was about 150ku, and 50% of the target protein was soluble. The purity could reach 95% after purified by Amylose Column. This study applies a basis for the development of diagnostic antigen and subunit vaccine against porcine pleuropneumonia.

关 键 词： 胸膜肺炎放线杆菌 融合表达 蛋白纯化

领　　域： [农业科学] [农业科学] [农业科学]