作　　者： ; ; ; ; (桑文彦）;

机构地区： 黑龙江八一农垦大学食品学院

出　　处： 《黑龙江八一农垦大学学报》 2011年第5期50-53,共4页

摘　　要： 利用MRS选择培养基筛选开菲尔粒中具有抑菌作用的乳酸菌。采用牛津杯琼脂扩散法,结合对理化抗性特征的鉴定、抗菌谱测定、菌落形态与特征和生理生化实验鉴定,结合伯杰氏手册对分离到的菌株进行鉴定。生理生化反应为乳酸菌,菌落形态与特征为球菌,对多数的革兰氏阴性菌有抑制作用,该菌株为乳酸乳球菌二乙酰乳酸亚种。鉴定分离为产球菌样细菌素的乳酸菌,产生的是蛋白肽类的细菌素。 To screen lactic acid bacteria of bacteriostasis using MRS selection culture medium.The agar diffusion method with Oxford cup methord was adopted,combining physical and chemical resistance of the characteristics identification,antibacterial spectrum determination colony morphology and characteristics,physiological and biochemical experiment,and the berger＇s manual identification were exercised.The physiological and biochemical reaction was lactic acid.The bacteria colony morphology and characteristics were Coccidia and have inhibition for most of the gram negative bacteria,this strain was lactic acid milk aureus the diacetyl lactic acid subspecies.Identification and separation results was in the bacteriocins coccoid lactic acid bacteria,and the bacteriocin was the like-protein peptide.

关 键 词： 开菲尔粒 球菌样细菌素 抑菌机理 分离 鉴定

领　　域： [农业科学] [农业科学]