作　　者： ; ; ; ; ; ; ; ;

机构地区： 南京师范大学生命科学学院江苏省生物多样性与生物技术重点实验室

出　　处： 《湖泊科学》 2011年第6期955-960,共6页

摘　　要： 利用植物组织培养技术培养水花生愈伤组织并以此为实验材料,研究了不同Cr6+浓度(0、0.1、0.3、0.6、0.9 mmol/L)对水花生愈伤组织中腐胺(putrescine,Put)、亚精胺(spermidine,Spd)、精胺(spermine,Spm)、脯氨酸(Pro)含量及精氨酸脱羧酶(ADC)、鸟氨酸脱羧酶(ODC)、多胺氧化酶(PAO)和二胺氧化酶(DAO)活性的影响.结果表明,随着Cr6+处理浓度的增大:(1)游离态腐胺(f-Put)、亚精胺(f-Spd)和精胺(f-Spm)的含量先升后降,且均在0.6 mmol/L时达到峰值;(2)结合态腐胺(结合态Put)含量在0.1 mmol/L时达到最大值,之后下降;而结合态Spd和结合态Spm含量则逐渐上升;(3)束缚态Put、束缚态Spd和束缚态Spm含量先升后降,在0.6 mmol/L时束缚态Put与Spd达到最大值;而束缚态Spm则是在0.1 mmol/L时达到最大值,之后逐渐下降;(4)ADC酶和ODC酶活性逐渐上升;而PAO、DAO酶活性则表现为先升后降;(5)Pro含量呈先升后降趋势.可见,重金属Cr6+胁迫下,破坏了水花生愈伤组织内多胺代谢平衡,造成多胺(polyamines,PAs)大量积累,较高的内源PAs在提高愈伤组织抵抗Cr6+胁迫能力的过程中发挥重要作用. Callus of Alternanthera philoxeroides,as experimental material,was cultured by tissue culture technique.In this study,the calluses were subjected to different concentrations of Cr6＋（0,0.1,0.3,0.6 and 0.9 mmol/L） and the impacts on contents of putrescine（Put）,spermidine（Spd）,spermine（Spm）and proline（Pro）,activities of arginine decarboxylase（ADC）,ornithine decarboxylase（ODC）,polyamine oxidase（PAO） and Diamine Oxidase（DAO） were investigated.Results indicated that,with the increasing of Cr6＋ concentration,（1） the contents of three free PAs increased at first and then decreased,and reached their peak values at 0.6 mmol/L Cr6＋ treatments;（2） the content of PS-conjugated Put reached its peak value at 0.1 mmol/L Cr6＋ treatment and then decreased slowly,while the contents of PS-conjugated spermidine（Spd） and spermine（Spm） increased significantly;（3） the peak values of PIS-conjugated Put and Spd were present at 0.6 mmol/L Cr6＋ treatment;meantime the peak value of PIS-conjugated Spm was present at 0.1 mmol/L Cr6＋ treatment;（4）the activities of ADC and ODC increased gradually,while PAO and DAO activities decreased sharply after a transient increase;（5） the level of Pro increased at 0.1-0.3 mmol/L Cr6＋ treatments and decreased at 0.6-0.9 mmol/L Cr6＋ treatments.These results suggested that Cr6＋ stress disturbed metabolic balance of polyamines and increased the level of PAs.Obviously,the changes of PAs levels might be essential to Cr6＋ adaption of cells.

关 键 词： 愈伤组织 水花生 胁迫 多胺 代谢

领　　域： [生物学]