机构地区: 佛山科学技术学院生命科学学院动物医学系
出 处: 《佛山科学技术学院学报(自然科学版)》 2011年第4期44-46,75,共4页
摘 要: 根据猪β干扰素基因序列设计一对引物,并按酵母密码子的偏好性,在引物中对稀有密码子进行同义突变,即第3位的TAT基因突变为TAC,第7位的CGA基因突变为AGA,第164位的CGG基因突变为CGT。同时,利用聚合酶链式反应基因定点突变技术,把17位的半胱氨酸密码子TGT突变为丝氨酸密码子TCT,构建poIFNβSer17突变体,然后将突变体克隆到pP ICZαC质粒中。结果表明,通过上述方法获得的poIFNβSer17突变体,并成功构建poIFNβSer17-pP ICZαC重组酵母表达质粒。 A pair of primer was designed according to Porcine interferon beta gene sequence recorded in GenBank.According to codon bias of yeast,the 3rd(TAT),7th(CGA)and 164th(CGG) codon was mutated to(TAC),(AGA),(CGT)respectively.And the 17th cysteine(TGT) was mutated to Serine(AGT).The IFN-β Ser17 which was amplified with PCR technique was cloned into expression vector pPICZαC.The results indicated that the IFN-β Ser17 mutant was obtained through PCR technique and the pPICZαC-IFN-β Ser17 recombinant expression vector was successfully constructed.