作　　者： ; ; ; ; ; ; (赵刚）; (王玉梅）;

机构地区： 中国检验检疫科学研究院

出　　处： 《寄生虫与医学昆虫学报》 2011年第3期184-188,共5页

摘　　要： 对2007年中俄联合监测小组在中俄边境7个口岸地区捕获的鼠进行病原体检测，以了解中俄边境鼠传疾病病原体携带情况。采用RT．PCR法检测鼠肺标本中的汉坦病毒RNA。PCR方法检测鼠肝脏、脾脏中的鼠疫杆菌、土拉杆菌、莫氏立克次氏体的DNA核酸。PCR方法检测肾脏中钩端螺旋体DNA核酸。ELISA方法检测血清中出血热病毒总抗体。胶体金法检测鼠疫杆菌抗体、钩端螺旋体抗体、土拉杆菌抗体、英氏立克次氏体抗体。结果显示鼠疫杆菌抗体、钩端螺旋体抗体、土拉杆菌抗体、立克次体抗体检测结果均为阴性。鼠疫杆菌、土拉杆菌、立克次体，钩端螺旋体的DNA核酸检测结果均为阴性。汉坦病毒3例核酸阳性，出血热总抗体8例阳性。结果表明中国抚远、俄罗斯布市、哈巴3个口岸地区应该注意肾综合征出血热的防控。 Investigation of rodent-borne pathogens was performed in seven ports on the borders between China and Russia for finding out the situation of rodent-borne pathogens. RT-PCR was employed to detect Hantavirus RNA from rodent＇s lung tissues, and PCR was used to detect the DNA of Yersinia pestis, Francisella tularensi and R. mooseri from rodents＇ liver or spleen, and also the DNA of Leptospira from rodents＇ kidney. Total antibody of haemorrhagic fever virus was detected by ELISA. Colloidal gold was used to detect the antibodys of Yersinia pestis, Leptospira, Francisella tularensi and R. mooseri. All the results of DNA or RNA or antibodies, detected by PCR or RT-PCR, of Yersinia pestis, Leptospira, Francisella tularensi and R. mooseri, were negative. However, 3 cases in nucleic acid detection and 8 cases in total antibody detection of hacmorrhagic fever virus were positive. Hemorrhagic fever should be of notice at Fuyuan port of China and Bushi and Haba ports of Rassia.

关 键 词： 中俄 双边 口岸 鼠传染病 病原体 检测

领　　域： [农业科学] [农业科学]