机构地区: 内蒙古农业大学动物科学学院
出 处: 《生物技术通报》 2011年第10期222-226,共5页
摘 要: 旨在研究转基因豆粕经挤压膨化后外源蛋白的变化规律。以CP4-EPSPS多克隆抗体和转基因含量为2%的大豆标准品作为材料,建立ELISA法定量检测抗草甘膦转基因大豆CP4-EPSPS蛋白的方法。结果表明,膨化豆粕中外源蛋白含量随着温度和含水率的升高逐渐降低,ELISA法检测低限达到0.25%,可以定量检测经过加工的转基因豆粕。 Variation tendency of CP4-EPSPS protein in genetically modified(GM) soybean via extrusion was tested in this study.The indirect ELISA assay was used to determine the CP4-EPSPS protein content with polyclonal antibody.An ELISA standard curve was created,and the LOD of ELISA was 0.25 %.The results indicated that the content of CP4-EPSPS protein decreased gradually with the increasing of temperature and moisture.Therefore,this method could be applied to detect the processed GM soybean.
领 域: [农业科学]