作　　者： ; ; ; ; ; ;

机构地区： 华南农业大学

出　　处： 《华南农业大学学报》 2011年第4期82-85,共4页

摘　　要： 利用mRNA差异显示技术对广东省地方品种蓝塘猪和引进品种大白猪达到经济成熟时的眼肌组织mRNA进行比较分析,并对差异显示条带回收产物进行PCR扩增,检测到与肉质性状相关的5条ESTs在2个猪种中存在表达差异,其中ESTsp7在大白猪种中表达量高,而ESTsp8、ESTsp9、ESTsp10和ESTsp11在蓝塘猪中表达量高于大白猪.通过NCBI检索发现,ESTsp7序列与细胞色素c氧化酶亚单位III基因在473 bp的范围内相似性达到100%,ESTsp9、ESTsp10和ESTsp11与猪SLN(Sarcolipin)基因相似性达到了94%～95%,而ESTsp8搜索不到相似基因. Comparative mRNA expression analysis of the ophthalmoplegia was made between Lantang （one of the Chinese indigenous pig breeds） and Large White using mRNA differential display technology. Differential expression bands were recycled and re-amplified. Different expressions of five ESTs s which were related to the meat quality traits were detected in the two pig breeds. ESTsp7 was expressed higher whereas ESTsp8, ESTsp9, ESTsp10 and ESTBoH were expressed lower in the Large White. Through the NCBI, it was found that ESTsp7 and cytochrome oxidase subunit m gene in the range of 473 bp had 100% similarity. ESTsp9, EST sp10, ESTsp11 and pig＇s SLN （Sarcolipin） gene had 94% - 95% similarity. ESTsp8 was not matched with any other genes.

关 键 词： 猪 差异显示技术 肉质性状

领　　域： [农业科学] [农业科学]