作　　者： ; ; ; ; ; ;

机构地区： 广东省农业科学院

出　　处： 《动物营养学报》 2011年第5期799-806,共8页

摘　　要： 本试验旨在通过研究饲喂氧化鱼油对新生仔猪生长性能、肠道黏膜氧化相关指标、免疫因子、氧化和免疫共同相关因子的影响以及添加大豆异黄酮(SI)对其的干预作用,探讨氧化应激对新生仔猪生长、肠道免疫应答的影响机制。试验选用4日龄新生仔猪96头,分成4组,每组6个重复,每个重复4头仔猪,各组饲粮中分别添加5%新鲜鱼油(Ⅰ组)、5%新鲜鱼油+50mg/kg SI(Ⅱ组)、5%氧化鱼油(Ⅲ组)和5%氧化鱼油+50mg/kg SI(Ⅳ组)。试验期21d。试验结束后,每重复选取2头仔猪进行屠宰取样。结果表明:试验第21天Ⅲ组仔猪体重显著低于Ⅱ组(P<0.05);1～21天Ⅲ组仔猪平均日增重显著低于Ⅰ组和Ⅱ组(P<0.05)。Ⅲ组仔猪肠道黏膜丙二醛(MDA)含量比Ⅰ组和Ⅱ组分别提高了90.89%(P<0.05)和73.28%(P<0.05)。SI添加显著提高肠道黏膜总超氧化物歧化酶(T-SOD)活性(P<0.05)。氧化鱼油和SI对肠道黏膜总抗氧化能力(T-AOC)产生极显著互作效应(P<0.01)。Ⅲ组新生仔猪肠道黏膜白细胞介素-2(IL2-)含量极显著高于Ⅰ组(P<0.01),白细胞介素-10(IL1-0)含量极显著低于Ⅰ组(P<0.01),白细胞介素-8(IL8-)含量显著低于Ⅰ组(P<0.05)。氧化鱼油显著提高了新生仔猪肠道黏膜诱导型一氧化氮合成酶(iNOS)活性(P<0.05),极显著提高了核因子-κB(NFκ-B)含量(P<0.01)。综上所述,饲粮中添加氧化鱼油显著降低了新生仔猪(4～24日龄)肠道抗氧化能力,引发了新生仔猪肠道的炎症反应。饲粮中添加SI在一定程度上可以缓解新生仔猪肠道受损。 This study was conducted to determine the effects of dietary oxidized fish oil（OFO） and the intervention of soybean isoflavones（SI） supplementation on growth performance,intestinal mucosal oxidation-related indicators,immune factors,and co-oxidation and immune-related factors in neonatal piglets,and to explore the effects of oxidative stress on growth and intestinal immune response in neonatal piglets.The neonatal piglets（n=96,4 day old） were selected and divided into four groups.They were fed 5% fresh fish oil（FFO）（group Ⅰ）,5% FFO＋50 mg/kg SI（group Ⅱ）,5% OFO（group Ⅲ）,OFO＋50 mg/kg SI（group Ⅳ）.After 21 d of feeding,two piglets per replicate were slaughtered,and small intestines were sampled.The results were showed as follows： at the end of 21 d,body weight of the neonatal piglets in group Ⅲ was significantly decreased compared with that in group Ⅱ（P0.05）.From 1 to 21 d,daily weight gain of the neonatal piglets in group Ⅲ was significantly lower than that in group Ⅰ and group Ⅱ（P0.05）.Compared with group Ⅰ and group Ⅱ,the content of malondialdehyde（MDA） in the intestinal mucosa of piglets in group Ⅲ was increased by 90.89%（P0.05） and 73.28%（P0.05）,respectively.SI supplementation significantly improved the activity of total superoxide dismutase（T-SOD） in intestinal mucosa（P0.05）.The OFO and SI produced a significant interaction effect on the activity of total anti-oxidation capability（T-AOC） in intestinal mucosa（P0.01）.The content of interleukin-2（IL-2） in group Ⅲ was significantly higher than the that in group Ⅰ（P0.01） and the contents of interleukin-8（IL-8）（P0.05） and interleukin-10（IL-10）（P0.01） in group Ⅲ were significantly lower than those in group Ⅰ（P0.05）,respectively.The OFO significantly increased the activity of inducible nitric oxide synthase（iNOS）（P0.05） and the content of nuclear factor-κB（NF-κB）（P0.01）.Collectively,these results suggest that dietary OFO

关 键 词： 新生仔猪 氧化鱼油 大豆异黄酮 小肠损伤

领　　域： [农业科学] [农业科学] [农业科学]