作　　者： ;

机构地区： 广东海洋大学农学院

出　　处： 《广东海洋大学学报》 2011年第4期17-22,共6页

摘　　要： 通过绿僵菌属甘露糖6-磷酸异构酶基因保守核苷酸区域设计简并性引物,采用RT-PCR及RACE-PCR技术成功克隆了金龟子绿僵菌mpi基因cDNA序列。该基因cDNA序列全长为1 513 bp,开放阅读框长度为1 328 bp,共编码441氨基酸。BLAST分析发现该基因演绎的氨基酸序列与其它真菌同源性较高,蛋白结构分析表明MPI蛋白是较保守的蛋白磷酸酶结构特征,主要由α螺旋和不规则卷曲构成。 The primers were designed according to the conservative sequence of mannose-6-phosphate isomerase gene,and the complete cDNA sequence of Metarhizium anisopliae was amplified by RT-PCR and RACE PCR.The complete cDNA sequence of mpi gene was 1513 bp and the complete ORF length was 1328 bp which encoded a protein with 441 amino acid residues.Blast analysis indicated the deduced amino acid sequence of the mpi gene from M.anisopliae showed high homology with other fungi respectively.The analysis of protein structure showed MPI protein had the characteristic of the conservative phosphate enzyme,and mainly constructed by α-helix and random coil.

关 键 词： 金龟子绿僵菌 甘露糖 磷酸异构酶基因 克隆

领　　域： [生物学]