作　　者： ; ; ; ; ; ; ; ;

机构地区： 南京农业大学无锡渔业学院

出　　处： 《生态学报》 2011年第17期4866-4873,共8页

摘　　要： 实验旨在探讨急性低温应激对吉富罗非鱼(GIFT,Oreochromis niloticus)血清生化、免疫指标以及肝脏HSP70 mRNA水平的影响。实验选取平均体重为(177±2.18)g左右的吉富罗非鱼作为实验对象,设定(25±1)℃对照组和低温(9±1)℃冷应激试验组,每组设定5个平行组,分别在0、2、6、12h随机采样,测定血清血糖(GLU)、胆固醇(CHOL)、甘油三酯(TG)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、补体3(C3)、补体4(C4)、溶菌酶(LSZ)、皮质醇(COR)、三碘甲腺原氨酸(T3)、甲状腺素(T4)以及肝脏HSP70mRNA水平。结果表明:与对照组相比,试验组GLU水平在冷应激后6h,TG水平在冷应激后2、6h,CHOL水平在冷应激后6、12h均有显著升高(P<0.05),ALT水平在冷应激后2、12h均有显著升高(P<0.05);试验组AST、COR、T3水平在冷应激2、6、12h时出现显著升高(P<0.05),C3、C4、LSZ水平在冷应激后2、6、12h均出现显著下降(P<0.05);与对照组相比,试验组HSP70mRNA水平在冷应激后的12h出现显著升高(P<0.05)。因此,急性低温应激可以提高吉富罗非鱼肝脏应激蛋白HSP70mRNA水平,影响该鱼的非特异免疫力和相关生理指标。 Genetic Improvement of Farmed Tilapia（GIFT,Oreochromis niloticus） is the genetically improved tilapia obtained by gene improvement performed by World Fish Research Centre and Norwegian related organization.The GIFT grows fast,is genetically stable and can tolerate low temperature of 6-10℃ for short periods,and tolerate temperature as high as 35-42℃.Tilapia is extremely hardy but may be stressed by excessively high or low temperatures which increase susceptibility to pathogens.Stress is the sum of an organism′s nonspecific responses to various acute external and internal stimuli.It is an organism′s physiological response to adapt to environmental changes,but excess response that inhibits immunity could lead to pathological change.In fish,excess stress can lead fish to diseased state,even to death.Cold temperature is an important ecological stressor,potentially leading to activation of the neuroendocrine pathway,including the hypothalamic-pituitary-interrenal axis,the hypothalamic-pituitary-thyroid axis,or the hypothalamus-autonomic nerves-chromaffin axis,increasing the release of cortisol,thyroid hormone,or catecholamines in vertebrates.Previous studies showed that cold stress resulted in altered serum lysozyme,complement activity and other related immune parameters in gilthead sea bream（Sparus aurata）,and altered serum biochemical parameters of milkfish（Chanos chanos） such as glucose and triglyceride.It also changed activities of alanine amiotransferase（ALT） and aspartate transaminase（AST） of common carp（Cyprinus carpio） and large yellow croaker（Pseudosciaena crocea）.Additionally,a study reported that cold stress increased the heat shock protein 70（HSP70） level in Atlantic salmon（Salmo salar）.Information about the relationship among the changes of serum hormone（triiodothyronine,thyroxine and cortisol） concentrations,serum nonspecific complement（C3 and C4） and serum biochemical parameters（such as serum glucose,cholesterol,triglyceride,aspartate aminotransferase

关 键 词： 吉富罗非鱼 低温应激 血液生理生化指标 基因表达

领　　域： [生物学]