作　　者： ; ; ; ;

机构地区： 华南农业大学

出　　处： 《北方园艺》 2011年第16期158-160,共3页

摘　　要： 以西南桦60d龄无菌苗茎、根、叶为外植体,研究不同种类和浓度的植物生长调节剂对西南桦愈伤组织诱导的影响。结果表明:当TDZ浓度为0.05mg/L时诱导效果较好。BA 1.5mg/L+NAA 0.2mg/L组合愈伤组织鲜重较高,KT 1.5mg/L+NAA 0.2mg/L的激素组合诱导的愈伤组织质量高,大部分愈伤组织表面疏松,灰白色,并有大量再生根缠绕。叶外植体用于愈伤组织诱导培养比茎、根外植体效果好。生长曲线表明,最佳的继代培养时间为45d。 Leaf,root and stem explants were taken from six-months-old in vitro grown plants of Betula alnoides B,Callus inducing was studied under culture media with different kinds and concentrations of hormones.The results indicated that 0.05 mg/L TDZ was suitable for the induction of callus.The highest weight had got with BA 1.5 mg/L＋NAA 0.2 mg/L.Calluses were enlarged and rooting with KT 1.5 mg/L＋NAA 0.2 mg/L,on this condition the callus grew well and offered the foundation of further study.The leaf explant was better than stem and root for callus induction.Growth curve of callus showed that the best time of subculture was about 45 days.

关 键 词： 西南桦 组织培养 生长曲线

领　　域： [农业科学] [农业科学]