作　　者： ; ; ;

机构地区： 仲恺农业工程学院园艺园林学院

出　　处： 《广西植物》 2011年第4期541-544,共4页

摘　　要： 为提高姜花属种间杂交胚挽救中幼胚萌发率,以白姜花×金姜花的胚珠为试材,研究不同胚珠发育时期、不同培养基及低温处理果实对幼胚萌发率的影响。结果表明,白姜花×金姜花胚挽救的适宜培养基是MS＋0.1 mg/L BA＋0.1 mg/L NAA;接种时期以60 d的幼胚培养效果最佳;低温处理果实3~6 d能有效提高幼胚的萌发率。 The object of this study was to improve the germination capacity of embryoes of Hedychium hybrids through embryo rescue in vitro.Hybrid ovules of H.coronarium×H.gardnerianum were used to investigate the effect of different growth stage of embryo,different culture medium and low-temperature treatment on the germination capacity of embryoes.The results indicated that MS medium supplemented with 0.1 mg/L BA and 0.1 mg/L NAA showed suitable for embryo rescue in vitro culture.It was showed that the optimum germination capacity of embryoes reached 40.6%,which was obtained by taking 60 day old immature embryo as explant.Low-temperature treatment of fruits for 3-6 d,had a positive effect on improvement of the germination capacity of embryoes.

关 键 词： 姜花属 种间杂交 胚拯救

领　　域： [生物学]