作　　者： ; ; ; ; ;

机构地区： 华南农业大学资源环境学院

出　　处： 《华中农业大学学报》 2011年第5期594-598,共5页

摘　　要： 由尖孢镰孢菌古巴专化型(Fusarium oxysporumf.sp.cubense)4号生理小种(Foc 4)引起的香蕉枯萎病是世界性香蕉毁灭性病害之一,毒素是其重要的致病因子。为了获得香蕉枯萎病菌毒素,采用活体香蕉苗生物测定法观察了不同培养基、培养方式、培养时间、培养温度、Richard培养基初始pH值、摇床转速、接种量和光照等条件对Foc 4产生毒素的影响。结果表明:Foc 4的最佳产生毒素条件为初始pH值7～9的Richard培养基,接种量为每300mL培养基5～9个菌丝块,培养温度25～30℃,24h持续光照,于转速140r/min摇床上振荡培养9～12d。 Banana fusarium wilt,caused by Fusarium oxysporum f.sp.cubense race 4（Foc 4）,is one of the most destructive diseases of banana worldwide.To obtain the toxin of Foc 4 effectively and provide the foundation for further research,the culture filtrates of Foc 4 obtained from different culture media,in different incubation modes,with different incubation time,incubation temperatures,initial pH values of Richard medium,rotation speeds of shaker,and inoculation amounts,under different illumination conditions were tested for their bioactivities to banana plantlets in vivo by using root-dipping method.An optimized system for toxin production of Foc 4 was established,in which the optimal condition for Foc 4 to produce toxin was obtained when it was cultured in Richard medium with an initial pH 7-9 and 5 mycelial plugs per 300 mL,in an incubation temperatures of 25-30 ℃ under continuous illumination for 24 h in a shaker at 140 r/min for 9-12 days.

关 键 词： 香蕉枯萎病菌 毒素 产毒条件 生物活性

领　　域： [农业科学] [农业科学] [农业科学] [农业科学]