作　　者： ; ; ; ; ; ; (黄科林）; (孙果宋）; (吴志洪）;

机构地区： 广西大学化学化工学院

出　　处： 《化工学报》 2011年第9期2588-2594,共7页

摘　　要： 引言 蚕蛹是养蚕业主要的副产物,蚕蛹蛋白质量占干蚕蛹质量的45%～50%。蚕蛹蛋白含有18种氨基酸,其中8种人体必需氨基酸含量占氨基酸总量40%以上,是一种优质蛋白源。文献报道蚕蛹蛋白酶解后的多肽具有抗氧化、降血压、抗疲劳、提高免疫力和抑制癌细胞扩散等作用。 The protein of silkworm pupae（PSP）is known for its nutritional value.The hydrolysates of PSP contained bioactive peptides with important bioactive activities,but enzymatic hydrolysis kinetics of PSP was less reported.This study applied lumping kinetics to the PSP-alcalase system.Solid-state PSP was the main substrate and soluble PSP was hardly responsive to alcalase.The molecular weight distribution of hydrolysates of solid-state PSP was analyzed by high performance size-exclusion chromatography（HPSEC）and the principle of lumping kinetics was built based on solubility of PSP and molecular weight distribution of hydrolysates.The lumped components of the complex reaction system were defined and a three-lumping reaction network was proposed.Reaction of the lumps was described with intrinsic kinetic equations,which contained product inhibition,substrate inhibition,enzyme inactivation and the variation of different lumped components in different times,and the system of differential equations for this reaction of lumps were established.The rate constant of each lump reaction was estimated by the Marquardt method.The reliability of the model was verified by comparing the computed values with the experimental values,and the average regression error was less than 5%.

关 键 词： 集总动力学 蚕蛹蛋白 碱性蛋白酶 酶解

领　　域： [生物学] [化学工程]